Aflatoxin B1 Induces Immunotoxicity through the DNA Methyltransferase-Mediated JAK2/STAT3 Pathway in 3D4/21 Cells
| العنوان: | Aflatoxin B1 Induces Immunotoxicity through the DNA Methyltransferase-Mediated JAK2/STAT3 Pathway in 3D4/21 Cells |
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| المؤلفون: | Xuan Zhou, Lili Hou, Fang Gan, Zixuan Liu, Kehe Huang, Guannan Le, Jiarui Su, Ziman Lin |
| المصدر: | Journal of Agricultural and Food Chemistry. 67:3772-3780 |
| بيانات النشر: | American Chemical Society (ACS), 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0106 biological sciences, Regulation of gene expression, DNA damage, Chemistry, 010401 analytical chemistry, General Chemistry, medicine.disease_cause, 01 natural sciences, DNA methyltransferase, Molecular biology, 0104 chemical sciences, Apoptosis, DNA methylation, DNMT1, medicine, General Agricultural and Biological Sciences, Carcinogenesis, Oxidative stress, 010606 plant biology & botany |
| الوصف: | As the most toxic mycotoxin of all of the fungal toxins, aflatoxin B1 (AFB1) has carcinogenesis, heptotoxicity, and immunotoxicity. DNA methylation plays a critical role in gene expression regulation of the pathological process. However, the relationship between DNA methylation and AFB1-induced immunotoxicity was not yet reported. Therefore, the objectives of this study were to verify AFB1-induced immunotoxicity and investigate the potential role of the DNA methyltransferase (DNMT) family in AFB1-induced immunotoxicity and the pathway mechanism in 3D4/21 cells. The results showed that AFB1 could induce cytotoxicity, apoptosis, pro-inflammatory cytokine expression, DNA damage, and oxidative stress and decrease phagocytotic capacity. Meanwhile, the levels of DNMT1 and DNMT3a were significantly increased in 0.04 and 0.08 μg/mL AFB1 compared to the control. Inhibition of DNMT1 and DNMT3a by 5-Aza-2dc could reverse changes of the above parameters. Further, the JAK2/STAT3 pathway was significantly activated in 0.04 μg/mL AFB1. Inhibition of p-JAK2 and p-STAT3 by AG490 could alleviate AFB1-induced immunotoxicity. Moreover, inhibition of DNMT1 and DNMT3a by 5-Aza-2dc could suppress the phosphorylation of JAK2 and STAT3. Taken together, AFB1-induced immunotoxicity is related to the JAK2/STAT3 pathway mediated by DNMTs in 3D4/21 cells. |
| تدمد: | 1520-5118 0021-8561 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::4d8c296ba4e0da98ba3d5eebad090f0e https://doi.org/10.1021/acs.jafc.8b07309 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........4d8c296ba4e0da98ba3d5eebad090f0e |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 15205118 00218561 |
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