Fasciola hepatica Fatty Acid Binding Protein (Fh12) inhibits the activation of murine dendritic cells
| العنوان: | Fasciola hepatica Fatty Acid Binding Protein (Fh12) inhibits the activation of murine dendritic cells |
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| المؤلفون: | Caleb Ruiz-Jiménez, Daiana Celias, Leonardo Silvane, Laura Cervi, Ana M. Espino |
| المصدر: | The Journal of Immunology. 198:68.15-68.15 |
| بيانات النشر: | The American Association of Immunologists, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Immunology, Immunology and Allergy |
| الوصف: | Fasciola hepatica, is a helminth that excretes-secretes different proteins that modulate cells of the immune system, generating anti-inflammatory responses. It has been demonstrated that Fh12 achieves this anti-inflammatory effect by inhibiting the expression of TLR4 induced by LPS in macrophages. Fh12 target the CD14 co-receptor, thus blocking the LPS-CD14 binding, which stop the entire TLR4 activation cascade from the beginning of the LPS-stimuli. Concurrently, Fh12 suppress the phosphorylation of various kinases (p38, JNK and ERK) downstream the TLR4 signaling cascade and induce the activation of macrophages by an alternative pathway. Dendritic cells (DCs) are antigen-presenting cells that play a key role at early phase of innate immunity and that are essential in the development of adaptive immune response. The main objective of the current study was to investigate the effect of Fh12 on the activation of murine DCs matured in the presence of LPS. DCs were isolated from bone marrow of naïve C57BL/6 mice, cultured, differentiated and stimulated in vitro with Fh12 1h prior to stimulation with LPS for 18 hrs. ELISA was used to quantify the amount of a panel of secreted cytokines in culture supernatant. FACS was used to determine the activation of MHCII and co-stimulatory molecules. Results demonstrated that Fh12 significantly inhibits the production of IL-12 and IL-6 and reduced the expression of MHCII and co-stimulatory molecules like CD80 on DCs surface. All assays have a significant P value < 0.05. These results suggest that Fh12 exerts a strong suppressive effect on activation of DC cells, which could have relevant implications in the subsequent development of adaptive immune response to microbial pathogens. |
| تدمد: | 1550-6606 0022-1767 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::4dfb61ceec934b1cf5e2e516359dd956 https://doi.org/10.4049/jimmunol.198.supp.68.15 |
| رقم الأكسشن: | edsair.doi...........4dfb61ceec934b1cf5e2e516359dd956 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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