A versatile system of high-flow high performance liquid chromatography with tandem mass spectrometry for rapid direct-injection analysis of plasma samples for quantitation of a β-lactam drug candidate and its open-ring biotransformation product
| العنوان: | A versatile system of high-flow high performance liquid chromatography with tandem mass spectrometry for rapid direct-injection analysis of plasma samples for quantitation of a β-lactam drug candidate and its open-ring biotransformation product |
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| المؤلفون: | Zheng Ouyang, Mark L. Powell, Yuan-Qing Xia, Mohammed Jemal |
| المصدر: | Rapid Communications in Mass Spectrometry. 13:1462-1471 |
| بيانات النشر: | Wiley, 1999. |
| سنة النشر: | 1999 |
| مصطلحات موضوعية: | Electrospray, Chromatography, Chemistry, Elution, Formic acid, Organic Chemistry, Tandem mass spectrometry, Mass spectrometry, High-performance liquid chromatography, Analytical Chemistry, Standard curve, chemistry.chemical_compound, Liquid chromatography–mass spectrometry, Spectroscopy |
| الوصف: | A bioanalytical method has been developed and validated for quantitation of a beta-lactam drug candidate and its open-ring biotransformation product utilizing high-flow high-performance liquid chromatography (HPLC) for on-line purification of plasma samples and electrospray tandem mass spectrometry for detection and quantitation. The HPLC system used two columns: an Oasis column (1 x 50 mm, 30 microm) as the on-line extraction column and a conventional C18 column (2 x 50 mm, 5 microm) as the analytical column. Each plasma standard or quality control (QC) sample (50 microL) was mixed with 50 microL of a working solution of the internal standard in aqueous 0.5 M ammonium acetate (pH 4.0). Portions (10 microL) of these samples were then injected into an Oasis column with a mobile phase consisting of 100% aqueous 1 mM formic acid at a high flow rate (4.0 mL/min), with the effluent from the Oasis column directed to waste and not to the mass spectrometer. After the purification step, the Oasis column effluent was directed to the analytical column and the mass spectrometer and the analytes were eluted with methanol/aqueous 1 mM formic acid (70:30) at a flow rate of 1.0 mL/min. The total analysis time was 1.6 min per sample. The standard curve range was 0.980 to 250 ng/mL. The accuracy, inter-day precision and intra-day precision were within 10% for both compounds. |
| تدمد: | 1097-0231 0951-4198 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::4e0c4fe672986ccbd62a552cfbf1f574 https://doi.org/10.1002/(sici)1097-0231(19990730)13:14<1462::aid-rcm668>3.0.co;2-z |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........4e0c4fe672986ccbd62a552cfbf1f574 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970231 09514198 |
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