A novel tumor necrosis factor-responsive transcription factor which recognizes a regulatory element in hemopoietic growth factor genes
| العنوان: | A novel tumor necrosis factor-responsive transcription factor which recognizes a regulatory element in hemopoietic growth factor genes |
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| المؤلفون: | Elizabeth Kuczek, S M Dunn, M F Shannon, M J Lenardo, F S Occhiodoro, L M Pell, Mathew A. Vadas |
| المصدر: | Molecular and Cellular Biology. 10:2950-2959 |
| بيانات النشر: | Informa UK Limited, 1990. |
| سنة النشر: | 1990 |
| مصطلحات موضوعية: | Upstream activating sequence, Sp1 transcription factor, GATA6, Sp3 transcription factor, biology.protein, Promoter, Cell Biology, Biology, Enhancer, Molecular Biology, Molecular biology, Transcription factor, Activating transcription factor 2 |
| الوصف: | A conserved DNA sequence element, termed cytokine 1 (CK-1), is found in the promoter regions of many hemopoietic growth factor (HGF) genes. Mutational analyses and modification interference experiments show that this sequence specifically binds a nuclear transcription factor, NF-GMa, which is a protein with a molecular mass of 43 kilodaltons. It interacts with different affinities with the CK-1-like sequence from a number of HGF genes, including granulocyte macrophage colony-stimulating factor (GM-CSF), granulocyte (G)-CSF, interleukin 3 (IL-3), and IL-5. We show here that the level of NF-GMa binding is induced in embryonic fibroblasts by tumor necrosis factor-alpha (TNF-alpha) treatment and that the CK-1 sequence from the G-CSF gene is a TNF-alpha-responsive enhancer in these cells. The NF-GMa protein is distinct from another TNF-alpha-responsive transcription factor, NF-kappa B, by several criteria. Firstly, several NF-kappa B-binding sites, although having sequence similarity with the CK-1 sequence, cannot compete efficiently for NF-GMa binding to CK-1. Secondly, the CK-1 sequence from both G-CSF and GM-CSF does not respond to phorbol ester treatment as would an NF-kappa B-binding element. These results demonstrate that NF-GMa is a novel transcription factor inducible by TNF-alpha and binds to a common element in HGF gene promoters. |
| تدمد: | 1098-5549 0270-7306 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::51e8d5be4b8f0aca71c576d6da11bc32 https://doi.org/10.1128/mcb.10.6.2950 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........51e8d5be4b8f0aca71c576d6da11bc32 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 10985549 02707306 |
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