The phosphorylation kinetics of the key regulators in T cell receptor signaling pathway
| العنوان: | The phosphorylation kinetics of the key regulators in T cell receptor signaling pathway |
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| المؤلفون: | Wei An, Kyueon Park, Alfonso Reyes, Amanda Kwieraga, Nazila Sabri, Avery Frey, Efthalia Chronopoulou, Jan Savas-Carstens, David Soper, Jeanne Elia, Michael Li, Xifeng Yang |
| المصدر: | The Journal of Immunology. 202:184.6-184.6 |
| بيانات النشر: | The American Association of Immunologists, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Immunology, Immunology and Allergy |
| الوصف: | T cell receptor (TCR) signaling pathway regulates T cell homeostasis and adaptive immune responses. Clinically, the activation of TCR is indispensable to the success of T cell-based immunotherapy. The engagement of TCR phosphorylates immunoreceptor tyrosine-based activation motifs (ITAM) on CD3, which is followed by a protein phosphorylation cascade. Protein phosphorylation, regulated by kinases and phosphatases, changes protein conformation and determines protein function. A better understanding of TCR signaling phosphorylation events is crucial to both decipher the T cell regulation mechanism and boost the efficacy of T cell immunotherapy. Here, we developed a panel of monoclonal antibodies that target several of the TCR signaling regulators and are applicable for both intracellular flow cytometry (ICFC) and western blot (WB). Using these antibodies, we performed a kinetic analysis of the TCR signaling pathway. Jurkat T cells were cross-linked by soluble anti-CD3 and anti-CD28 antibodies, and the phosphorylation kinetics of the key TCR signaling regulators, such as phospho-ZAP-70 and phospho-PLCg1, were evaluated by ICFC and WB. We show that TCR signaling activation induced by cross-linking CD3 and CD28 is a relatively transient event and the optimal activation of the key TCR signaling regulators varies from 1 to 15 minutes. Our data profiled the phosphorylation kinetics of the key regulators in TCR signaling pathway. |
| تدمد: | 1550-6606 0022-1767 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::52c9e58b9f9ad0a8e79ada67bd2276b3 https://doi.org/10.4049/jimmunol.202.supp.184.6 |
| رقم الأكسشن: | edsair.doi...........52c9e58b9f9ad0a8e79ada67bd2276b3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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