Mating-Type Genes Control Sexual Reproduction, Conidial Germination, and Virulence in Cochliobolus lunatus
| العنوان: | Mating-Type Genes Control Sexual Reproduction, Conidial Germination, and Virulence in Cochliobolus lunatus |
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| المؤلفون: | Jiaqi Jia, Dandan Fu, Jiamei Zhao, Jiaying Sun, Shuqin Xiao, Chunsheng Xue, Jiayang Li, Nan Chen, Kexin Liu |
| المصدر: | Phytopathology®. 112:1055-1062 |
| بيانات النشر: | Scientific Societies, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Mating type, biology, Curvularia, Botany, Leaf spot, Virulence, Plant Science, Pathogenic fungus, biology.organism_classification, Cochliobolus lunatus, Agronomy and Crop Science, Cochliobolus, Sexual reproduction |
| الوصف: | Cochliobolus lunatus (anamorph: Curvularia lunata) is a major pathogenic fungus that causes the Curvularia leaf spot of maize. ClMAT1-1-1 and ClMAT1-2-1, the C. lunatus orthologs of C. heterostrophus ChMAT1-1-1 and ChMAT1-2-1, were investigated in the present study to uncover their functions in C. lunatus. Southern blot analysis showed that these mating-type MAT genes exist in the C. lunatus genome as a single copy. ClMAT1-1-1 and ClMAT1-2-1 were knocked out and complemented to generate ΔClmat1-1-1 and ΔClmat1-2-1 and ΔClmat1-1-1-C and ΔClmat1-2-1-C, respectively. The mutant strains had defective sexual development and failed to produce pseudothecia. There were no significant differences in growth rate or conidia production between the mutant and wild-type strains. However, the aerial mycelia and mycelial dry weight of ΔClmat1-1-1 and ΔClmat1-2-1 were lower than those of wild type, suggesting that MAT genes affect asexual development. ClMAT genes were involved in the responses to cell wall integrity and osmotic adaptation. ΔClmat1-2-1 had a lower conidial germination rate than the wild-type strain CX-3. The virulence of ΔClmat1-2-1 and ΔClmat1-1-1 was also reduced compared with the wild-type. Complementary strains could restore all the phenotypes. |
| تدمد: | 1943-7684 0031-949X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::53346264a42e28bd1de3130f825fcd48 https://doi.org/10.1094/phyto-02-21-0063-r |
| رقم الأكسشن: | edsair.doi...........53346264a42e28bd1de3130f825fcd48 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19437684 0031949X |
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