A pH-responsive high density lipoprotein-like nanoparticle of epothilone B
| العنوان: | A pH-responsive high density lipoprotein-like nanoparticle of epothilone B |
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| المؤلفون: | Chol-Su Shin, Jichun Lee, Jonghyeok Shin, Young-Ha Song, Heekyung An, Jung-Su Oh, Dae-Hyuk Kweon, Woo-Jong Lee, Sung-Gun Kim, Byoungjae Kong, Keejung Yoon, Jae Yoon Shin, Jae Youl Cho |
| المصدر: | Biotechnology and Bioprocess Engineering. 20:249-258 |
| بيانات النشر: | Springer Science and Business Media LLC, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Endosome, Biomedical Engineering, Bioengineering, Applied Microbiology and Biotechnology, chemistry.chemical_compound, Paclitaxel, chemistry, Biochemistry, Cell culture, Apoptosis, Cancer cell, Cancer research, Scavenger receptor, Cytotoxicity, Biotechnology, Lipoprotein |
| الوصف: | Epothilone B (EpoB) is a paclitaxel (PTX)-like microtubule-stabilizing agent that induces mitotic arrest and apoptosis in cells. EpoB is a promising anti-tumor agent, and is thought to have the potential to overcome well-known PTX resistance. However, EpoB is barely soluble in water, and is fatal to normal cells due to extremely potent cytotoxicity. To reduce the unwanted cytotoxicity of EpoB to normal cells, a reconstituted highdensity lipoprotein (rHDL)-like nanoparticle of EpoB (EpoB-rHDL) was assembled using apolipoprotein A-I (apoA-I). The EpoB-rHDL, as well as PTX-rHDL (a HDLlike nanoparticle of paclitaxel), were indeed mild (nontoxic) to certain cell lines such as MCF7, MDA-MB-231, and SK-OV-3, while free EpoB and PTX were very toxic to those same cells. In contrast, the EpoB-rHDL and PTXrHDL were very effective in killing the Caco-2 and ZR-75-1 while free drugs were less toxic to those cells. The susceptibility of cell lines to rHDLs was dependent on the expression of scavenger receptor class B type I (SR-BI), indicating that EpoB-rHDL selectively and efficiently kills only SR-BI-overexpressing cells. Furthermore, the EpoBrHDL released EpoB only at acidic pH, which may facilitate the escape of drugs from acidic endosome. Thus, EpoB-rHDL shown in this study enables safe and targeted delivery of the potent EpoB to cancer cells in SR-BIdependent manner. |
| تدمد: | 1976-3816 1226-8372 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::55ea8cdffdb40782df07dbd8b320f82d https://doi.org/10.1007/s12257-014-0368-y |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........55ea8cdffdb40782df07dbd8b320f82d |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 19763816 12268372 |
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