The aim of this research was to study the effects of dietary supplementation on the composition and fatty acid (FA) profile of milk from grazing dairy sheep. Forty-eight lactating Sarda ewes were randomly allocated to four homogeneous groups: PAS – control group grazing for 22 h/d without supplementation; NFS – group supplemented with a cereal based concentrate; SLNA – group supplemented with a concentrate enriched with sunflower seeds (106 g/kg DM); and SALA, group supplemented with a concentrate enriched with linseeds (105 g/kg DM). Milk from groups receiving the oilseed enriched concentrate (SLNA and SALA) showed increased total C18:2 non-conjugated dienes, C18:1 t 9 and total trans C18:1 by 106% (P c9, t11 , (2.90 vs 2.27 g/100 g of FAME; P c9, t11 in milk, also resulted in some counters effects on milk nutritional quality, compared to grazing without supplementation.