Strain improvement ofArthrobacter simplex by protoplast fusion
| العنوان: | Strain improvement ofArthrobacter simplex by protoplast fusion |
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| المؤلفون: | Lo Ck, L.-W. Chow, W.-H. Liu |
| المصدر: | Journal of Industrial Microbiology. 16:257-260 |
| بيانات النشر: | Oxford University Press (OUP), 1996. |
| سنة النشر: | 1996 |
| مصطلحات موضوعية: | Cell fusion, Strain (chemistry), Cholesterol oxidase, Auxotrophy, Mutant, Bioengineering, Protoplast, Biology, Applied Microbiology and Biotechnology, chemistry.chemical_compound, Biochemistry, chemistry, Cholesterol oxidase activity, Lysozyme, Biotechnology |
| الوصف: | Anl-tryptophan auxotroph and milky mutants were derived from an inducible cholesterol oxidase-producing bacterium,Arthrobacter simplex USA18, via UV-mutagenesis. Protoplasts of these mutants and a constitutive cholesterol oxidase producer, strain US3011, were prepared by growing cells in the presence of ampicillin (20μg ml−1) followed by digestion with lysozyme. Protoplast fusion between tested strains with complementary characteristics was achieved in the presence of 20–40% polyethylene glycol 6000. The fusion frequency was about 1.5–1.7×10−3. The cholesterol oxidase activity of four fusants in a cholesterol-containing medium was 20–60% higher than that of parental strains. This study demonstrated that protoplast fusion is applicable to strain improvement ofArthrobacter strains for enzyme production. |
| تدمد: | 1476-5535 0169-4146 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::5b31c3d060e5975d6730099ec7333cf1 https://doi.org/10.1007/bf01570030 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........5b31c3d060e5975d6730099ec7333cf1 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 14765535 01694146 |
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