In a two-lactation-gestation cycle experiment, 152 Holstein cows with low serum Se and vitamin E were fed total mixed rations and assigned at parturition to four groups (1, control; 2, 500 IU vitamin E/d; 3, 2mg Se/d; 4, 500 IU vitamin E plus 2mg Se/d). Supplements were not fed during dry periods. Serum Se and vitamin E were increased within 1 mo by oral supplements. Maximal mean serum Se in cycles 1 (67ng/ml at 7 mo) and 2 (74ng/ml at 4 mo) occurred in groups 3 and 4, respectively. Maximal mean serum vitamin E in cycle 1 (3.3 μ g/ml at mo 8) and 2 (3.03 μ g/ml at mo 2) occurred in groups 4 and 2, respectively. Selenium treatment of the dams increased Se in colostrum and in serum of presuckle calves. Vitamin E supplementation of dams did not affect vitamin E in serum of presuckled calves. Reproductive performance was not affected by supplement. In an 8-wk study, 24 lactating cows with low serum Se were assigned (6/group) to 0, 2.5, 5, and 10mg supplemental Se/d. Maximal mean serum Se concentrations of 23, 56, 71, and 79ng/ml were attained by wk 4 in the above respective groups. These data indicate that 2 to 2.5mg supplemental Se/cow per d were inadequate for desirable serum Se concentrations and support recent changes in allowed Se supplementation for dairy cattle.
