AB0984 CHANGES IN PLASMA VOLUME WHEN MEASURING BIOCHEMICAL MARKERS OF JOINT TISSUE TURNOVER IN RELATION TO ACUTE PHYSICAL ACTIVITY
| العنوان: | AB0984 CHANGES IN PLASMA VOLUME WHEN MEASURING BIOCHEMICAL MARKERS OF JOINT TISSUE TURNOVER IN RELATION TO ACUTE PHYSICAL ACTIVITY |
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| المؤلفون: | J. Bjerre-Bastos, C. Sejersen, H. B. Nielsen, N. Secher, C. C. Kitchen, C. P. Miller, A. Mackey, A. R. Bihlet |
| المصدر: | Annals of the Rheumatic Diseases. 81:1618.1-1618 |
| بيانات النشر: | BMJ, 2022. |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Rheumatology, Immunology, Immunology and Allergy, General Biochemistry, Genetics and Molecular Biology |
| الوصف: | BackgroundPhysical activity can induce acute changes in plasma volume (PV) and thereby influence concentration of plasma constituents, such as biochemical markers1,2. However, it remains undocumented to what extent PV changes occur during physical exercise in osteoarthritis (OA) patients.ObjectivesInvestigating the direct course and magnitude of PV changes during and after weight-bearing (WB) and non-weight-bearing (NWB) exercise, adrenaline-induced cardiovascular stress and resting in an OA population.MethodsData originates from the EFEX-OA-02 study (Reg. no.: NCT04542668), which explored biomarker changes in knee OA patients during and after running, cycling, adrenaline infusion and resting on separate days. Blood samples were obtained before, during, at five time points after and again 24 hours post exercise/infusion. Hemoglobin (Hgb) and derived hematocrit (Hct) were measured at all time-points to monitor PV fluctuations.Main inclusion criteria: Cumulative Kellgren-Lawrence (KL) radiographic grade of the left and right knee of ≥ 2, 40-75 years of age, bodyweight 50-100 kg and BMI 18.5-35.0 kg/m2.Active interventions consisted of 4x5-minute intervals each progressing from low intensity and peaking at ≥80% of the heart rate reserve (HRR). For the adrenaline infusion, 0.06 mg/kg of adrenalin was prepared in 50 mL saline and administered intravenously. At rest, blood samples were collected at time points similar to the other study interventions, however the 24-hour follow-up sample was omitted.Hgb was measured on a ABL800 FLEX blood gas analyzer and converted to Hct using the formula: Hct (%) = (0.0485 × ctHb (mmol/L) + 0.0083) × 100. Estimated PV change (% ΔPV) was calculated as: % ΔPV = [100 / (100 – Hctpre)] x [100 (Hctpre – Hctpost) / Hctpost], where: ΔPV % = Percentage change in PV: Hctpre = Hct before exercise, Hctpost = Hct after exercise. A repeated measures linear mixed-model and 1-way ANOVA was used to assess PV changes and compare interventions, respectively.ResultsForty subjects were included. Mean age was 60.4 years (Standard deviation (SD): 8.7), 16 (40%) were male, mean BMI was 27.0 kg/m2 (SD: 3.5), and mean score in the nine item pain domain of the Knee Injury and Osteoarthritis Outcome Score (KOOS) at baseline was 67.5 (SD: 15.2) out of 100 (0 and 100 corresponding to max pain and no pain, respectively). Baseline Hgb was 9.05 mmol/L (SD: 0.68) and Hct was 44.7% (SD: 2.9%). All subjects were able to reach the defined peak cardiovascular intensity or higher during exercise, while reaching on average 70% (SD: 8.7%) of that during the adrenaline infusion.Cycling, running and adrenaline infusion led to acute reductions in PV compared to rest: Cycling -14.3% (95%CI: -10.0 to -18.7%), running -13.9% (95%CI: -10.9 to -17.0%), adrenaline -7.8% (95%CI: -4.2 to -11.5%). The reductions in PV induced by both cycling and running were greater compared to adrenaline (pConclusionModerate-high intensity running, cycling and cardiovascular stress led to rapid reductions in PV of knee OA patients. Adjustment for PV changes should be considered when measuring biochemical markers in relation to physical activity.References[1]Kaltreider NL, Meneely GR: THE EFFECT OF EXERCISE ON THE VOLUME OF THE BLOOD. J Clin Invest, 1940.[2]Novosadová J: The changes in hematocrit, hemoglobin, plasma volume and proteins during and after different types of exercise. Eur J Appl Physiol Occup Physiol, 1977.Disclosure of InterestsJonathan Bjerre-Bastos: None declared, Casper Sejersen: None declared, Henning Bay Nielsen: None declared, Niels Secher: None declared, Carl-Christian Kitchen: None declared, Claire Prener Miller: None declared, Abigail Mackey: None declared, Asger Reinstrup Bihlet Employee of: Full-time employee of NBCD/Sanos Group A/S |
| تدمد: | 1468-2060 0003-4967 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::5c467294ce08c29c23c6ccf97173606d https://doi.org/10.1136/annrheumdis-2022-eular.2774 |
| رقم الأكسشن: | edsair.doi...........5c467294ce08c29c23c6ccf97173606d |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14682060 00034967 |
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