Binding preferential of chickpea cold shock protein during nucleic acid interactions
| العنوان: | Binding preferential of chickpea cold shock protein during nucleic acid interactions |
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| المؤلفون: | Yadav Bs, Swati Singh, Pratibha Bhadauriya, Ashutosh Mani |
| المصدر: | Journal of Plant Biochemistry and Biotechnology. 26:208-215 |
| بيانات النشر: | Springer Science and Business Media LLC, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, 030103 biophysics, Multiple sequence alignment, RNA, Plant Science, Biology, Cold-shock domain, 03 medical and health sciences, 030104 developmental biology, Biochemistry, Docking (molecular), Complementary DNA, GenBank, Nucleic acid, Agronomy and Crop Science, Peptide sequence, Biotechnology |
| الوصف: | Cold shock proteins (CSPs) have a widespread occurrence from prokaryotes to eukaryotes including plants. These proteins are known to possess nucleic acid binding properties. CSPs have a single cold shock domain in prokaryotes while N-terminal and C-terminal flanking regions are present in eukaryotic CSPs. The objective of this study was to investigate nucleic acid binding preferential for the chickpea CSP. Full cDNA of chickpea CSP was cloned and sequenced. The sequence was submitted to GenBank (accession no. KM036036) at NCBI. Multiple sequence alignment and phylogenetic analysis further revealed that the inferred amino acid sequence belongs to CSP family. Molecular docking was performed between the CSP and variety of nucleic acids entities. These results suggest that CSPs of chickpea possess preferential binding affinity for single stranded nucleic acids. Docking results suggest that homo-polymer entities of RNA polyU RNA (20mer) form most stable complex. |
| تدمد: | 0974-1275 0971-7811 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::600a7f11456c6034654a4f3cff58a10e https://doi.org/10.1007/s13562-016-0383-5 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........600a7f11456c6034654a4f3cff58a10e |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 09741275 09717811 |
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