Detection and Partial Purification of a Natural Heparin Inhibitor from Hog Small Intestine
| العنوان: | Detection and Partial Purification of a Natural Heparin Inhibitor from Hog Small Intestine |
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| المؤلفون: | Mingjien Chien, Robert H. Yue, Menard M. Gertler |
| المصدر: | Thrombosis and Haemostasis. 41:567-575 |
| بيانات النشر: | Georg Thieme Verlag KG, 1979. |
| سنة النشر: | 1979 |
| مصطلحات موضوعية: | Ammonium sulfate, Ethanol, Chemistry, Precipitation (chemistry), chemistry.chemical_element, Hematology, Heparin, Zinc, Small intestine, chemistry.chemical_compound, medicine.anatomical_structure, Biochemistry, medicine, Imidazole, Specific activity, medicine.drug |
| الوصف: | SummaryA natural occurring heparin inhibitor was detected and was partially purified from the mucosa of hog small intestine. The mucosa was homogenized and was extracted overnight in 0.15 M NaCl, 0.01 M imidazole, 0.001 M EDTA, pH 6.5. When the extract was made to 85% saturation in ammonium sulfate, a large quantity of heparin neutralizing activity was detected in the precipitate. Each small intestine contains approximately 35,000 units of heparin neutralizing activity. This heparin inhibitor was further purified by the procedures of zinc sulfate precipitation, ammonium sulfate fractionation, ethanol precipitation and heparin-sepharose chromatography. A 37 fold partial purification with 15% overall recovery was achieved to yield heparin inhibitor with specific activity of 50-65 units per mg of protein. |
| تدمد: | 2567-689X 0340-6245 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::63e09b9eaf0f04d5a7e7c6fbc096351c https://doi.org/10.1055/s-0038-1646809 |
| رقم الأكسشن: | edsair.doi...........63e09b9eaf0f04d5a7e7c6fbc096351c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 2567689X 03406245 |
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