Intracellular Storage and Regulated Plasma Membrane Expression of Human Complement Receptor Type 1 in Rat Basophil Leukemia Cell Transfectants
| العنوان: | Intracellular Storage and Regulated Plasma Membrane Expression of Human Complement Receptor Type 1 in Rat Basophil Leukemia Cell Transfectants |
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| المؤلفون: | Melvin Berger, Anoopa Kumar, Erica M. Wetzler, Carolina Jost, Lloyd Klickstein |
| المصدر: | Blood. 92:300-309 |
| بيانات النشر: | American Society of Hematology, 1998. |
| سنة النشر: | 1998 |
| مصطلحات موضوعية: | Vesicle, Immunology, hemic and immune systems, Cell Biology, Hematology, Complement receptor, Biology, Biochemistry, Secretory Vesicle, Cell biology, Cell membrane, medicine.anatomical_structure, Complement Receptor Type 1, medicine, Secretion, Cell activation, Intracellular |
| الوصف: | Polymorphonuclear neutrophils (PMN) contain multiple distinct secretory compartments that are sequentially mobilized during cell activation. Complement receptor type 1 (CR1) is a marker for a readily mobilizable secretory vesicle compartment, which can undergo exocytic fusion with the plasma membrane independently of secretion of traditional granule contents. The basis for the formation of these distinct compartments is incompletely understood. Primary and secondary granules are generated directly from the Golgi complex during different stages of development of the cell, obviating the need for sorting signals for proper packaging of their constituents. To determine whether the secretory vesicles are formed in a similar manner, we studied a stable rat basophilic leukemia cell line (RBL-CR1) transfected with a plasmid containing the cDNA of human CR1 driven by a viral promoter. The CR1 was present primarily intracellularly in small vesicles resembling the CR1 storage pools in resting PMN. Activation of RBL-CR1 resulted in translocation of intracellular CR1 to the plasma membrane, with mobilization requirements different from those of the classical RBL granules. Thus, in RBL-CR1, continuously synthesized CR1 is stored and upregulated in much the same way as in PMN. This suggests that differential timing of gene expression is not essential for proper storage of CR1 and that other sorting mechanisms are involved, which can be studied in RBL-transfectants. |
| تدمد: | 1528-0020 0006-4971 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::641815f2e3ce9c2d0dbcfb46b15d8663 https://doi.org/10.1182/blood.v92.1.300.413k29_300_309 |
| رقم الأكسشن: | edsair.doi...........641815f2e3ce9c2d0dbcfb46b15d8663 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15280020 00064971 |
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