J-binding protein increases the level and retention of the unusual base J in trypanosome DNA
| العنوان: | J-binding protein increases the level and retention of the unusual base J in trypanosome DNA |
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| المؤلفون: | Robert Sabatini, Anita Dirks-Mulder, Piet Borst, Inês Chaves, Rudo Kieft, Mike Cross |
| المصدر: | Molecular Microbiology. 46:37-47 |
| بيانات النشر: | Wiley, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | biology, Binding protein, Base J, Trypanosoma brucei, biology.organism_classification, Microbiology, Molecular biology, Nuclear DNA, Thymine, chemistry.chemical_compound, chemistry, Biochemistry, Gene expression, Molecular Biology, Gene, DNA |
| الوصف: | Summary The nuclear DNA of Trypanosoma brucei and other kinetoplastid flagellates contains the unusual base β-d-glucosyl-hydroxymethyluracil, called J, replacing part of the thymine in repetitive sequences. We have described a 100 kDa protein that specifically binds to J in duplex DNA. We have now disrupted the genes for this J-binding protein (JBP) in T. brucei. The disruption does not affect growth, gene expression or the stability of some repetitive DNA sequences. Unexpectedly, however, the JBP KO trypanosomes contain only about 5% of the wild-type level of J in their DNA. Excess J, randomly introduced into T. brucei DNA by growing the cells in the presence of the J precursor 5-hydroxymethyldeoxyuridine, is lost by simple dilution as the KO trypanosomes multiply, showing that JBP does not protect J against removal. In contrast, cells containing JBP lose excess J only sluggishly. We conclude that JBP is able to activate the thymine modification enzymes to introduce additional J in regions of DNA already containing a basal level of J. We propose that JBP is a novel DNA modification maintenance protein. |
| تدمد: | 1365-2958 0950-382X |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::6488f21c373f17bb40b7085b2f433773 https://doi.org/10.1046/j.1365-2958.2002.03144.x |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........6488f21c373f17bb40b7085b2f433773 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652958 0950382X |
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