P- and L-selectin mediate distinct but overlapping functions in endotoxin-induced leukocyte-endothelial interactions in the rat mesenteric microcirculation
| العنوان: | P- and L-selectin mediate distinct but overlapping functions in endotoxin-induced leukocyte-endothelial interactions in the rat mesenteric microcirculation |
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| المؤلفون: | K L Davenpeck, D A Steeber, T F Tedder, B S Bochner |
| المصدر: | The Journal of Immunology. 159:1977-1986 |
| بيانات النشر: | The American Association of Immunologists, 1997. |
| سنة النشر: | 1997 |
| مصطلحات موضوعية: | Immunology, Immunology and Allergy |
| الوصف: | Endotoxin is a potent stimulus of leukocyte infiltration, but the adhesion-related mechanisms responsible for LPS-induced cell recruitment events in vivo remain poorly characterized. Utilizing intravital microscopy, we examined the role of P- and L-selectin in LPS-induced inflammation. We demonstrated that superfusion of rat mesentery with LPS resulted in significant increases in both leukocyte rolling and adherence, which were maintained for at least 2 h. Pretreatment with a P-selectin neutralizing mAb only partially inhibited LPS-induced leukocyte rolling, but completely inhibited LPS-induced leukocyte adherence throughout the 2-h observation period. Pretreatment with an L-selectin neutralizing mAb dramatically inhibited LPS-induced increases in leukocyte rolling, but unlike the P-selectin mAb did not inhibit leukocyte adhesion. Fucoidin, which blocks both P- and L-selectin function, completely inhibited LPS-induced leukocyte rolling and adhesion. Consistent with previous studies, leukocyte rolling velocities on P-selectin were observed to be far less than velocities observed for leukocytes rolling on L-selectin in vivo. These data suggest that P-selectin plays a role in LPS-induced rolling and is essential for LPS-induced leukocyte adherence, while L-selectin functions in LPS-induced rolling, but not in adhesion. |
| تدمد: | 1550-6606 0022-1767 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::66e95931c74eca02bc14e1852c1fe818 https://doi.org/10.4049/jimmunol.159.4.1977 |
| رقم الأكسشن: | edsair.doi...........66e95931c74eca02bc14e1852c1fe818 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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