Bacterial Strain Alcaligenes denitrificans C-32 Containing Two Nitrilases with Different Substrate Specificities
| العنوان: | Bacterial Strain Alcaligenes denitrificans C-32 Containing Two Nitrilases with Different Substrate Specificities |
|---|---|
| المؤلفون: | T. E. Leonova, S. A. Glinskii, L. E. Riabchenko, K. V. Lavrov, Andrey Novikov, A. S. Yanenko, G. A. Larikova |
| المصدر: | Applied Biochemistry and Microbiology. 53:786-791 |
| بيانات النشر: | Pleiades Publishing Ltd, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Alcaligenes faecalis, biology, Chemistry, Stereochemistry, 030106 microbiology, Acidovorax facilis, Alcaligenes denitrificans, Nucleic acid sequence, Benzyl cyanide, biology.organism_classification, Applied Microbiology and Biotechnology, Biochemistry, Nitrilase, Homology (biology), 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, Aliphatic nitrilase |
| الوصف: | Two genes encoding nitrilases with different properties have been found in an Alcaligenes denitrificans C-32 strain with high nitrilase activity that is currently used as a biocatalyst for commercial ammonium acrylate production. Both genes were expressed in E. coli, and the properties of the recombinant nitrilases were studied. One of these genes, which is designated as nitC1, controlled the formation of nitrilase that preferred aliphatic nitriles (acrylonitrile and butyronitrile) as best substrates. The nucleotide sequence of the gene nitC1 was almost (99%) identical to the gene sequence of an aliphatic nitrilase from Acidovorax facilis 72W (DQ4444267). In turn, nitC2 had a high level of homology (85%) with the arylacetonitrilase gene from Alcaligenes faecalis JM3 (D13419). Benzyl cyanide was shown to be the best substrate for nitC2-encoded nitrilase. In light of the results of DNA homology and differences in substrate specificity, the NitC2 and NitC1 nitrilases from Alcaligenes denitrificans C-32 were allocated to the groups of aliphatic nitrilases and arylacetonitrilases, respectively. |
| تدمد: | 1608-3024 0003-6838 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::683b3e59ed8eaa5718285507d3902a4d https://doi.org/10.1134/s0003683817080051 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........683b3e59ed8eaa5718285507d3902a4d |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 16083024 00036838 |
|---|