Tag's memory can be easily accessed by readers without any type of authentication technique in GEN2 protocol. In this paper, we use security implementation and mutual authentication between tag and reader of three different lightweight ciphers. We used Hummingbird (HB), PRESENT, and Extended Tiny Encryption Algorithm (XTEA) to encrypt the data and implemented all three algorithms to FPGA devices. We finally implemented PRESENT with PUFFIN as a trail and we got better results compared to the former three ciphers based on performance, data blocks and execution time.