A European inter-laboratory pre-validation of in vitro assays to evaluate immunotoxicity (47.18)
| العنوان: | A European inter-laboratory pre-validation of in vitro assays to evaluate immunotoxicity (47.18) |
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| المؤلفون: | Rob Vandebriel, Andre Kleensang, Lydia-Mareen Koeper, Armelle Biola-Vidamment, Danielle Fiechter, Maria Carfi, Laura Gribaldo, Emanuela Corsini, Marc Pallardy, Raymond Pieters, Hans-Werner Vohr, Henk Van Loveren |
| المصدر: | The Journal of Immunology. 182:47.18-47.18 |
| بيانات النشر: | The American Association of Immunologists, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | Immunology, Immunology and Allergy |
| الوصف: | The new European Union REACH (Registration, Evaluation and Authorisation of new and existing CHemicals) policy will increase the use of laboratory animals if alternative methods are not available. The immune system is a target for many chemicals and drugs, with potential adverse effects on human health. We performed an inter-laboratory pre-validation of a set of in vitro assays to detect immunosuppressive capacity. Human PBMC and murine and rat splenocytes were used. Six immunosuppressive compounds (benzo(a)pyrene, cyclophosphamide, methotrexate, rapamycin, dexamethasone, and urethane) and one non-immunosuppressive compound (D-mannitol) were tested. Basal cytotoxicity was assessed by measuring LDH-release. Human PBMC were stimulated with anti-CD3/anti-CD28, murine splenocytes with anti-CD3/anti-CD28, Con A, LPS, and anti-CD40/IL-4, and rat splenocytes with Con A and LPS. Cell proliferation and IFN-γ and TNF-α production were measured. Five out of 6 immunosuppressive compounds could be identified as such, and the non-immunotoxic one came out as negative. This suggests that in vitro assays are able to detect immunosuppressive capacity. The sensitivity of the various species/stimulant/endpoint combinations ranged between 38 and 61%, while the specificity ranged between 67 and 100%. This suggests that from these results a choice for specific species/stimulant/endpoint combinations cannot be made. |
| تدمد: | 1550-6606 0022-1767 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::6c1b8a8c28d9f309fa981dc60be240a9 https://doi.org/10.4049/jimmunol.182.supp.47.18 |
| رقم الأكسشن: | edsair.doi...........6c1b8a8c28d9f309fa981dc60be240a9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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