التفاصيل البيبلوغرافية
| العنوان: |
Platform technology to generate broadly cross-reactive antibodies to α-helical epitopes in hemagglutinin proteins from influenza A viruses |
| المؤلفون: |
Jonathan A. Shortt, David D. Pollock, Lajos Gera, Zhe Yan, Brooke Hirsch, Robert S. Hodges, Kathryn V. Holmes, Colin T. Mant, Zhaohui Qian, Ziqing Jiang |
| المصدر: |
Biopolymers. 106:144-159 |
| بيانات النشر: |
Wiley, 2016. |
| سنة النشر: |
2016 |
| مصطلحات موضوعية: |
0301 basic medicine, chemistry.chemical_classification, Immunogen, 030102 biochemistry & molecular biology, biology, Organic Chemistry, Biophysics, Hemagglutinin (influenza), General Medicine, medicine.disease_cause, Bioinformatics, Biochemistry, Virology, Influenza A virus subtype H5N1, Epitope, Virus, Conserved sequence, Biomaterials, 03 medical and health sciences, 030104 developmental biology, chemistry, Polyclonal antibodies, medicine, biology.protein, Glycoprotein |
| الوصف: |
We have utilized a de novo designed two-stranded α-helical coiled-coil template to display conserved α-helical epitopes from the stem region of hemagglutinin (HA) glycoproteins of influenza A. The immunogens have all the surface-exposed residues of the native α-helix in the native HA protein of interest displayed on the surface of the two-stranded α-helical coiled-coil template. This template when used as an immunogen elicits polyclonal antibodies which bind to the α-helix in the native protein. We investigated the highly conserved sequence region 421-476 of HA by inserting 21 or 28 residue sequences from this region into our template. The cross-reactivity of the resulting rabbit polyclonal antibodies prepared to these immunogens was determined using a series of HA proteins from H1N1, H2N2, H3N2, H5N1, H7N7, and H7N9 virus strains which are representative of Group 1 and Group 2 virus subtypes of influenza A. Antibodies from region 449-476 were Group 1 specific. Antibodies to region 421-448 showed the greatest degree of cross-reactivity to Group 1 and Group 2 and suggested that this region has a great potential as a "universal" synthetic peptide vaccine for influenza A. © 2016 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 106: 144-159, 2016. |
| تدمد: |
0006-3525 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_________::6d3295b0ed82c55ed5c00b419f1441d3
https://doi.org/10.1002/bip.22808 |
| حقوق: |
OPEN |
| رقم الأكسشن: |
edsair.doi...........6d3295b0ed82c55ed5c00b419f1441d3 |
| قاعدة البيانات: |
OpenAIRE |
