Karyotype Analysis and Chromosomal Distribution of Repetitive DNA Sequences of Cucumis metuliferus Using Fluorescence in situ Hybridization
| العنوان: | Karyotype Analysis and Chromosomal Distribution of Repetitive DNA Sequences of Cucumis metuliferus Using Fluorescence in situ Hybridization |
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| المؤلفون: | Magdalena Pawełkowicz, Wojciech Pląder, Ewa Siedlecka, Stefan Malepszy, Kouhei Yagi, Yoshikazu Hoshi, Michał Wojcieszek, Norikazu Tagashira, Zbigniew Przybecki |
| المصدر: | Cytogenetic and Genome Research. 144:237-242 |
| بيانات النشر: | S. Karger AG, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Genetics, medicine.diagnostic_test, Chromosome, Karyotype, Biology, biology.organism_classification, food.food, food, Chromosome 3, medicine, Molecular Biology, Cucumis metuliferus, Cucumis, Ribosomal DNA, Genetics (clinical), Chromosome 12, Fluorescence in situ hybridization |
| الوصف: | Cucumis metuliferus (2n = 24) is a cultivated species of the Cucumis genus which is a potential genetic resource for Cucumis crops. Although some cytogenetic research has been reported, there is no study of karyotyping in this species. Here, we used 4′,6-diamidino-2-phenylindole and chromomycin A3 staining to identify 12 pairs of chromosomes in early-metaphase cells. Fluorescence in situ hybridization revealed the chromosomal distribution patterns of the 5S and 45S ribosomal DNA (rDNA) genes, telomeres, and 3 different satellite repeats. The 2 major signals of the 45S rDNA were located on the satellite of chromosome 11, and the 2 signals of the 5S rDNA and 2 minor signals of the 45S rDNA were located on chromosome 12. The telomere probes hybridized to the ends of all chromosomes. The 3 satellite DNAs were localized at the ends of chromosomes 1, 2, 4-10, and at the end of the short arm of chromosome 3. In summary, we reported the identification of all chromosomes of C. metuliferus. We also depicted the location of 5S and 45S rDNA, the telomere motif sequence, CmetSat1, CmetSatT2, and CmetmSat1 in an ideogram. |
| تدمد: | 1424-859X 1424-8581 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::6d74b6405f2057b5419f8eb373f8d1ef https://doi.org/10.1159/000369183 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........6d74b6405f2057b5419f8eb373f8d1ef |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1424859X 14248581 |
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