التفاصيل البيبلوغرافية
| العنوان: |
The human kinesin-14 HSET tracks the tips of growing microtubules in vitro |
| المؤلفون: |
Marcus Braun, Seweryn Bajer, Stefan Diez, Andrzej A. Kasprzak, Gero Fink, Zdenek Lansky |
| المصدر: |
Cytoskeleton. 70:515-521 |
| بيانات النشر: |
Wiley, 2013. |
| سنة النشر: |
2013 |
| مصطلحات موضوعية: |
0303 health sciences, Microtubule dynamics, macromolecular substances, Cell Biology, Biology, biology.organism_classification, In vitro, 3. Good health, Cell biology, Motor protein, 03 medical and health sciences, 0302 clinical medicine, Structural Biology, Microtubule, Melanogaster, Molecular motor, Kinesin, Peptide sequence, 030217 neurology & neurosurgery, 030304 developmental biology |
| الوصف: |
Tip-tracking of kinesin-14 motor proteins is believed to be crucial for the assembly and maintenance of dynamic microtubule arrays. However, in contrast to other members of the kinesin-14 family, H. sapiens kinesin-14 HSET has so far never been observed to be prominently located at microtubule plus ends. Here, using an in vitro microtubule dynamics reconstitution assay we observe tip-tracking of GFP-HSET in the presence of H. sapiens EB1 (hsEB1). Tip-tracking depended on the SxIP-like motif in HSET as well as on the EB homology domain in hsEB1. D. melanogaster Ncd and S. pombe Klp2 tip-tracking reconstitution assays accompanied by kinesin-14 amino acid sequence comparisons suggest that SxIP-like motif mediated tip-tracking dependent on EB family proteins is conserved in the kinesin-14 family of molecular motors. © 2013 Wiley Periodicals, Inc. |
| تدمد: |
1949-3584 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_________::6e66f708f87714ce13eece006db66550
https://doi.org/10.1002/cm.21133 |
| حقوق: |
CLOSED |
| رقم الأكسشن: |
edsair.doi...........6e66f708f87714ce13eece006db66550 |
| قاعدة البيانات: |
OpenAIRE |
