The yeast ESCRT complexes are involved in the regulation of transcription elongation
| العنوان: | The yeast ESCRT complexes are involved in the regulation of transcription elongation |
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| المؤلفون: | Young-Ha Song, Ruxin Duan, Seong Hoon Ahn, Hong-Yeoul Ryu |
| المصدر: | Genes & Genomics. 36:335-343 |
| بيانات النشر: | Springer Science and Business Media LLC, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Endosome, Protein subunit, Saccharomyces cerevisiae, macromolecular substances, Biology, biology.organism_classification, Biochemistry, ESCRT, Cell biology, ESCRT complex, Membrane protein, Transcription (biology), Genetics, Molecular Biology, Gene |
| الوصف: | Endosomal sorting complex required for transport (ESCRT) is involved in membrane protein deg- radation through the recognition and sorting of ubiquity- lated cargo proteins into the multivesicular body before fusion with the lysosome/vacuole. However, recent studies have challenged this canonical cellular function of ESCRT and have implicated a role for this machinery in multiple intracellular pathways. Here, we provide evidence that ESCRT complexes contribute to the regulation of tran- scription elongation in Saccharomyces cerevisiae. Most strains deficient in each subunit of ESCRT-0, -I, -II, and - III showed significant sensitivity to 6-azauracil or myco- phenolic acid, a phenotype associated with transcription elongation defects. Moreover, these deletion strains sig- nificantly reduced transcription activation through Gcn4, a regulator of the general amino acid control. The tran- scription factor Rim101, which is proteolytically activated through the multimerized component Vps32/Snf7 of ESCRT-III and its collaborative proteins, was not associ- ated with transcription elongation or Gcn4 activation. In addition, we observed that ESCRT complexes were crosslinked at the 3 0 region of the coding sequence in the actively transcribed gene. In summary, these results sug- gest that ESCRT complexes promote genes transcription during the late stages of elongation and are required for transcription activation through Gcn4. |
| تدمد: | 2092-9293 1976-9571 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::6ef9a499533e8c3e1e07f8ff767b60b6 https://doi.org/10.1007/s13258-013-0171-8 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........6ef9a499533e8c3e1e07f8ff767b60b6 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 20929293 19769571 |
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