A Single Effector Protein, AvrRpt2EA, from Erwinia amylovora Can Cause Fire Blight Disease Symptoms and Induces a Salicylic Acid–Dependent Defense Response
| العنوان: | A Single Effector Protein, AvrRpt2EA, from Erwinia amylovora Can Cause Fire Blight Disease Symptoms and Induces a Salicylic Acid–Dependent Defense Response |
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| المؤلفون: | John L. Norelli, Erik H. A. Rikkerink, Christoph Böttcher, Thomas Wöhner, Magda-Viola Hanke, Susan Schröpfer, Henryk Flachowsky, Klaus Richter |
| المصدر: | Molecular Plant-Microbe Interactions®. 31:1179-1191 |
| بيانات النشر: | Scientific Societies, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, biology, Physiology, Effector, Jasmonic acid, fungi, food and beverages, General Medicine, Erwinia, Plant disease resistance, biology.organism_classification, 01 natural sciences, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, chemistry, Fire blight, Gene expression, Agronomy and Crop Science, Salicylic acid, Systemic acquired resistance, 010606 plant biology & botany |
| الوصف: | The AvrRpt2EA effector protein of Erwinia amylovora is important for pathogen recognition in the fire blight–resistant crabapple Malus × robusta 5; however, little is known about its role in susceptible apples. To study its function in planta, we expressed a plant-optimized version of AvrRpt2EA driven by a heat shock–inducible promoter in transgenic plants of the fire blight–susceptible cultivar Pinova. After induced expression of AvrRpt2EA, transgenic lines showed shoot necrosis and browning of older leaves, with symptoms similar to natural fire blight infections. Transgenic expression of this effector protein resulted in an increase in the expression of the salicylic acid (SA)-responsive PR-1 gene but, also, in the levels of SA and its derivatives, with diverse kinetics in leaves of different ages. In contrast, no increase of expression levels of VSP2 paralogs, used as marker genes for the activation of the jasmonic acid (JA)-dependent defense pathway, could be detected, which is in agreement with metabolic profiling of JA and its derivatives. Our work demonstrates that AvrRpt2EA acts as a virulence factor and induces the formation of SA and SA-dependent systemic acquired resistance. |
| تدمد: | 1943-7706 0894-0282 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::6f488d07dd735f9ae8eb1fa055868e8b https://doi.org/10.1094/mpmi-12-17-0300-r |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........6f488d07dd735f9ae8eb1fa055868e8b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19437706 08940282 |
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