Isolation of Aeromonas sobria JC18 from milkfish (Chanos chanos) intestine with proteolytic and cellulolytic activities for fish probiotic
| العنوان: | Isolation of Aeromonas sobria JC18 from milkfish (Chanos chanos) intestine with proteolytic and cellulolytic activities for fish probiotic |
|---|---|
| المؤلفون: | A F Rohman, I N Atitus, D D Heraswati, I Istiqomah, A Isnansetyo |
| المصدر: | IOP Conference Series: Earth and Environmental Science. 919:012055 |
| بيانات النشر: | IOP Publishing, 2021. |
| سنة النشر: | 2021 |
| الوصف: | Attention on the probiotic application for the improvement of fish resident intestinal microbiota has risen in the last decades. It is demonstrated that probiotics may function not only by direct inhibition of pathogenic bacteria or manipulation of enzymatic digestion of feed but also by modulation of immunity in fish. In the present study, we aimed to isolate the autochthonous microbiota of marine fish intestines as probiotic candidates based on cellulolytic and proteolytic activity. Bacteria were isolated and purified on a cellulose agar with 24h of incubation. A total of 18 bacterial strains were purified and stored in -80°C. Phenotypic screening based on the antibiotic resistance, antagonistic activity against pathogenic bacteria, resistance to an acidic environment, and ability of colonization in fish intestine found a selected strain, namely JC18. Infection test, molecular and phenotypic characterizations revealed that the JC18 isolate was a non-pathogenic Aeromonas sobria. It is hence revealed that the milkfish intestine is a potent source of proteolytic bacteria for fish probiotic screening. |
| تدمد: | 1755-1315 1755-1307 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::70a5b9f4df51af071d11dbf598076c17 https://doi.org/10.1088/1755-1315/919/1/012055 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........70a5b9f4df51af071d11dbf598076c17 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17551315 17551307 |
|---|