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Novel mouse models based on intersectional genetics to identify and characterize plasmacytoid dendritic cells

التفاصيل البيبلوغرافية
العنوان: Novel mouse models based on intersectional genetics to identify and characterize plasmacytoid dendritic cells
المؤلفون: Michael Valente, Nils Collinet, Thien-Phong Vu Manh, Dimitri Popoff, Khalissa Rahmani, Karima Naciri, Gilles Bessou, Rejane Rua, Laurine Gil, Cyrille Mionnet, Pierre Milpied, Elena Tomasello, Marc Dalod
المصدر: Nature Immunology. 24:714-728
بيانات النشر: Springer Science and Business Media LLC, 2023.
سنة النشر: 2023
مصطلحات موضوعية: Immunology, Immunology and Allergy
الوصف: Plasmacytoid dendritic cells (pDCs) are the main source of type I interferon (IFN-I) during viral infections. Their other functions are debated, due to a lack of tools to identify and target them in vivo without affecting pDC-like cells and transitional DCs (tDCs), which harbor overlapping phenotypes and transcriptomes but a higher efficacy for T cell activation. In the present report, we present a reporter mouse, pDC-Tom, designed through intersectional genetics based on unique Siglech and Pacsin1 coexpression in pDCs. The pDC-Tom mice specifically tagged pDCs and, on breeding with Zbtb46GFP mice, enabled transcriptomic profiling of all splenic DC types, unraveling diverging activation of pDC-like cells versus tDCs during a viral infection. The pDC-Tom mice also revealed initially similar but later divergent microanatomical relocation of splenic IFN+ versus IFN− pDCs during infection. The mouse models and specific gene modules we report here will be useful to delineate the physiological functions of pDCs versus other DC types.
تدمد: 1529-2916
1529-2908
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::739ab906d94afce38f086fdf17c3329a
https://doi.org/10.1038/s41590-023-01454-9
حقوق: OPEN
رقم الأكسشن: edsair.doi...........739ab906d94afce38f086fdf17c3329a
قاعدة البيانات: OpenAIRE
الوصف
تدمد:15292916
15292908