Characterization of Colibactin-Producing Escherichia coli Isolated from Japanese Patients with Colorectal Cancer
| العنوان: | Characterization of Colibactin-Producing Escherichia coli Isolated from Japanese Patients with Colorectal Cancer |
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| المؤلفون: | Fumi Higashiguchi, Hideki Ishikawa, Yuji Iwashita, Haruhiko Sugimura, Yuichiro Hirayama, Kenji Watanabe, Noriyuki Miyoshi, Nobuo Matsuzaki, Michihiro Mutoh, Yuta Tsunematsu, Keiji Wakabayashi, Yuko Yoshikawa, Michio Sato |
| المصدر: | Japanese Journal of Infectious Diseases. 73:437-442 |
| بيانات النشر: | Editorial Committee of Japanese Journal of Infectious Diseases, National Institute of Infectious Dis, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Microbiology (medical), biology, Colorectal cancer, 030106 microbiology, General Medicine, medicine.disease_cause, medicine.disease, biology.organism_classification, Molecular biology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Infectious Diseases, Biosynthesis, chemistry, Colibactin, medicine, Selective advantage, Colorectal adenocarcinoma, 030212 general & internal medicine, Carcinogenesis, Escherichia coli, Bacteria |
| الوصف: | We investigated the relationship between colibactin-producing (clb+) Escherichia coli and colorectal adenocarcinoma. In total, 729 E. coli colonies were isolated from tumor and surrounding non-tumor regions in resected specimens from 34 Japanese patients; 450 colonies were from the tumor regions and 279 from the non-tumor regions. clb+ bacteria were found in tumor regions of 11 patients (11/34, 32.4%) and they were also detected in the non-tumor regions of 7 out of these 11 patients (7/34, 20.6%). The prevalence of clb+ isolates was 72.7% (327/450) and 44.1% (123/279) in tumor and non-tumor regions, respectively. All the recovered clb+ isolates belonged to the phylogenetic group B2 and were the most predominant type in tumor regions. Hemolytic (α-hemolysin-positive, hlyA+) and non-hemolytic (α-hemolysin-negative, hlyA-) clb+ isolates were obtained from patient #19; however, the prevalence of hlyA+ clb+ isolates was significantly higher in tumor regions (35/43, 81.4%) than in non-tumor regions (3/19, 15.8%). Moreover, a significantly higher production of N-myristoyl-D-asparagine, a by-product of colibactin biosynthesis, was observed in hlyA+ clb+ isolates than in hlyA- clb+ isolates. Our results suggest that hlyA+ clb+ E. coli may have a selective advantage in colorectal colonization and, consequently, might play a role in carcinogenesis. The presence of hlyA+ clb+ bacteria in healthy individuals is a potential risk marker of colorectal cancer. |
| تدمد: | 1884-2836 1344-6304 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::7636c4b00cc65b2c38dbe1c3d3b87dbc https://doi.org/10.7883/yoken.jjid.2020.066 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........7636c4b00cc65b2c38dbe1c3d3b87dbc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18842836 13446304 |
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