Temperature and oxygen level determine N 2 O respiration activities of heterotrophic N 2 O‐reducing bacteria: Biokinetic study
| العنوان: | Temperature and oxygen level determine N 2 O respiration activities of heterotrophic N 2 O‐reducing bacteria: Biokinetic study |
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| المؤلفون: | Yiwen Zhou, Masaaki Hosomi, Shohei Riya, Toshikazu Suenaga, Akihiko Terada, Chuang Qi |
| المصدر: | Biotechnology and Bioengineering. 118:1330-1341 |
| بيانات النشر: | Wiley, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | biology, Chemistry, Heterotroph, Bioengineering, Nitrous oxide, biology.organism_classification, Applied Microbiology and Biotechnology, Pseudomonas stutzeri, chemistry.chemical_compound, Respiration, Food science, Paracoccus denitrificans, Azospira sp, Oxygen level, Bacteria, Biotechnology |
| الوصف: | Nitrous oxide (N2 O), a potent greenhouse gas, is reduced to N2 gas by N2 O-reducing bacteria (N2 ORB), a process which represents an N2 O sink in natural and engineered ecosystems. The N2 O sink activity by N2 ORB depends on temperature and O2 exposure, yet the specifics are not yet understood. This study explores the effects of temperature and oxygen exposure on biokinetics of pure culture N2 ORB. Four N2 ORB, representing either clade I type nosZ (Pseudomonas stutzeri JCM5965 and Paracoccus denitrificans NBRC102528) or clade II type nosZ (Azospira sp. strains I09 and I13), were individually tested. The higher activation energy for N2 O by Azospira sp. strain I13 (114.0 ± 22.6 kJ mol-1 ) compared with the other tested N2 ORB (38.3-60.1 kJ mol-1 ) indicates that N2 ORB can adapt to different temperatures. The O2 inhibition constants (KI ) of Azospira sp. strain I09 and Ps. stutzeri JCM5965 increased from 0.06 ± 0.05 and 0.05 ± 0.02 μmol L-1 to 0.92 ± 0.24 and 0.84 ± 0.31 μmol L-1 , respectively, as the temperature increased from 15°C to 35°C, while that of Azospira sp. strain I13 was temperature-independent (p = 0.106). Within the range of temperatures examined, Azospira sp. strain I13 had a faster recovery after O2 exposure compared with Azospira sp. strain I09 and Ps. stutzeri JCM5965 (p < 0.05). These results suggest that temperature and O2 exposure result in the growth of ecophysiologically distinct N2 ORB as N2 O sinks. This knowledge can help develop a suitable N2 O mitigation strategy according to the physiologies of the predominant N2 ORB. |
| تدمد: | 1097-0290 0006-3592 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::794d9724de9a06d513c4a632ee67198b https://doi.org/10.1002/bit.27654 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........794d9724de9a06d513c4a632ee67198b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970290 00063592 |
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