Isolation and identification of nitrifying bacteria from tilapia (Oreochromis sp.) pond in Sleman Yogyakarta Indonesia
| العنوان: | Isolation and identification of nitrifying bacteria from tilapia (Oreochromis sp.) pond in Sleman Yogyakarta Indonesia |
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| المؤلفون: | I Istiqomah, A Isnansetyo, I Aswiyanti |
| المصدر: | IOP Conference Series: Earth and Environmental Science. 919:012054 |
| بيانات النشر: | IOP Publishing, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Veterinary medicine, Oreochromis, food.ingredient, food, biology, Nitrifying bacteria, Tilapia, Identification (biology), Isolation (microbiology), biology.organism_classification |
| الوصف: | This research aims to isolate and identify autochtonous nitrifying bacteria from tilapia pond in Sleman Yogyakarta Indonesia for future application in aquaculture practices in the region. Bacteria were isolated using a nitrification medium. Bacterial characterization was carried out by non-pathogenic test to tilapia (Oreochromis sp.), and nitrification activity test in a single bacterial fermentation medium for 9 days. Bacterial identification was carried out based on the colony and cell morphologies, biochemical tests, and molecular analysis using the 16S rRNA and gyrB genes. A total of 15 isolates of nitrifying bacteria were obtained. Four non-pathogenic isolates obtained the highest nitrification activity on the sixth day of incubation, with nitrate production of 17.26-21.54 ppm. Two selected bacteria, isolates A2 and A3, have colony morphology that is milky white, smooth surface, circular shape, entire edge, and convex elevation. Both bacteria are short rods, Gram-negative, non-motile, produce catalase, fermenting glucose, sucrose, and lactose, and do not produce oxidase, ornithine decarboxylase, indole, and H2S. Molecular analysis showed that the two isolates had the highest similarity (99.28% and 99.34%) to Klebsiella spp. |
| تدمد: | 1755-1315 1755-1307 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::79c492a4af9bd6674954e0c425e683aa https://doi.org/10.1088/1755-1315/919/1/012054 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........79c492a4af9bd6674954e0c425e683aa |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17551315 17551307 |
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