Biocompatible carbon dots with low-saturation-intensity and high-photobleaching-resistance for STED nanoscopy imaging of the nucleolus and tunneling nanotubes in living cells
| العنوان: | Biocompatible carbon dots with low-saturation-intensity and high-photobleaching-resistance for STED nanoscopy imaging of the nucleolus and tunneling nanotubes in living cells |
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| المؤلفون: | Jiaqing Guo, Shuai Ye, Hao Li, Jun Song, Wei Yan, Junle Qu, Huibo Wang |
| المصدر: | Nano Research. 12:3075-3084 |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Photoluminescence, Materials science, business.industry, Resolution (electron density), STED microscopy, Nanoparticle, Quantum yield, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 01 natural sciences, Photobleaching, Fluorescence, Atomic and Molecular Physics, and Optics, 0104 chemical sciences, Optoelectronics, General Materials Science, Stimulated emission, Electrical and Electronic Engineering, 0210 nano-technology, business |
| الوصف: | Many kinds of nanoparticles and organic dyes as fluorescent probes have been used in the stimulated emission depletion (STED) nanoscopy. Due to high toxicity, photobleaching and non-water solubility, these fluorescent probes are hard to apply in living cell imaging. Here, we report a new fluorescence carbon dots (FNCDs) with high photoluminescence quantum yield (56%), low toxicity, anti-photobleaching and good water-solubility that suitable for live-cell imaging can be obtained by doping fluorine element. Moreover, the FNCDs can stain the nucleolus and tunneling nanotubes (TNTs) in the living cell. More importantly, for STED nanoscopy imaging, the FNCDs effectively depleted background signals and improved imaging resolution. Furthermore, the lateral resolution of single FNCDs size under the STED nanoscopy is up to 22.1 nm for FNCDs deposited on a glass slide was obtained. And because of their good water dispersibility, the higher resolution of single FNCDs size in the nucleolus of a living cell can be up to 19.7 nm. After the image optimization steps, the fine fluorescence images of TNTs diameter with ca. 75 nm resolution is obtained living cell, yielding a threefold enhancement compared with that in confocal imaging. Additionally, the FNCDs show excellent photobleaching resistance after 1,000 scan cycles in the STED model. All results show that FNCDs have significant potential for application in STED nanoscopy. |
| تدمد: | 1998-0000 1998-0124 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::7a52eb43614cbbec014392a56135cf0a https://doi.org/10.1007/s12274-019-2554-x |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........7a52eb43614cbbec014392a56135cf0a |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 19980000 19980124 |
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