Studies on Bioactive Marine Metabolite-XIII. Isolation and Characterization of a Protease Inhibitor from the Marine Gastropod Tugali gigas
| العنوان: | Studies on Bioactive Marine Metabolite-XIII. Isolation and Characterization of a Protease Inhibitor from the Marine Gastropod Tugali gigas |
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| المؤلفون: | Rina Goto, Hisao Kamiya, Koji Muramoto, Yoshikazu Yoshino |
| المصدر: | NIPPON SUISAN GAKKAISHI. 59:1923-1928 |
| بيانات النشر: | Japanese Society of Fisheries Science, 1993. |
| سنة النشر: | 1993 |
| مصطلحات موضوعية: | Serine protease, Proteases, biology, Kunitz STI protease inhibitor, Elastase, Fast protein liquid chromatography, Aquatic Science, Trypsin, Protease inhibitor (biology), Biochemistry, Enzyme inhibitor, biology.protein, medicine, medicine.drug |
| الوصف: | A protease inhibitor, named tugalistatin, was isolated from the muscle of the marine gastropod Tugali gigas. Tugalistatin inhibited the proteolytic activity of trypsin and α-chymotrypsin. It also suppressed the activity of kallikrein as well as elastase to some extent. On the other hand, it was inactive against metallo-proteases and cysteine proteases. These suggest that tugalistatin is a serine protease inhibitor. In SDS-polyacryamide gel electrophoresis it showed a single band corresponding to 22, 000 under reduced conditions. The molecular weight of intact inhibitor was calculated to be 17, 000 when checked by FPLC on a Superose 12 column. Amino acid analysis revealed tugalistatin contained a large amount of glutamic acid (14.0%), half-cystine (11.1%), and aspartic acid (9.7%) in the molecule. |
| تدمد: | 1349-998X 0021-5392 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::7c94e576b7bed799ee45416fbe7deadc https://doi.org/10.2331/suisan.59.1923 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........7c94e576b7bed799ee45416fbe7deadc |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1349998X 00215392 |
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