Measuring temporal expression, systematic response, and post-mortem stability to assess potential markers for estimating wound age: an example of Fosl1 in contused skeletal muscle
| العنوان: | Measuring temporal expression, systematic response, and post-mortem stability to assess potential markers for estimating wound age: an example of Fosl1 in contused skeletal muscle |
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| المؤلفون: | San-qiang Li, Ta-na Dong, Jun-hong Sun, Qiu-xiang Du, Xi-yan Zhu |
| المصدر: | Australian Journal of Forensic Sciences. 51:158-170 |
| بيانات النشر: | Informa UK Limited, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Pathology, medicine.medical_specialty, Pentobarbital, 010401 analytical chemistry, Chloral hydrate, Lethal dose, Skeletal muscle, Biology, FOSL1, 01 natural sciences, 0104 chemical sciences, Pathology and Forensic Medicine, Blot, 03 medical and health sciences, 0302 clinical medicine, Real-time polymerase chain reaction, medicine.anatomical_structure, Age estimation, medicine, 030216 legal & forensic medicine, medicine.drug |
| الوصف: | To assess whether Fosl1 is a suitable parameter for wound age estimation, a total of 126 male Sprague–Dawley rats were divided into control (n = 6 per group), contusion (including 4, 8, 12, 16, 20, 24, 28, 32, 36, 40, 44, and 48 h post-severe injury, and 4 or 8 h post-moderate or -mild injury subgroups), and post-mortem (including 6, 12, 18, and 24 h subgroups) groups (n = 6 per subgroup). A contusion was produced in the right limb of the rats using the drop-ball technique (under intraperitoneal chloral hydrate anaesthesia), and the animals were sacrificed using a lethal dose of pentobarbital. The expression of Fosl1 mRNA and protein was determined in the contused and contralateral uninjured muscle and post-mortem specimens of musculi quadriceps femoris using real-time PCR and western blotting, respectively. Compared with the control group, the expression of Fosl1 mRNA was increased in several contused and contralateral uninjured muscles, and decreased in post-mortem groups (P |
| تدمد: | 1834-562X 0045-0618 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::7e495aa2df23a473bbf4824427e68e70 https://doi.org/10.1080/00450618.2017.1334824 |
| رقم الأكسشن: | edsair.doi...........7e495aa2df23a473bbf4824427e68e70 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 1834562X 00450618 |
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