HPLC Analysis and Pharmacokinetics of Picroside I in Dog Plasma
العنوان: | HPLC Analysis and Pharmacokinetics of Picroside I in Dog Plasma |
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المؤلفون: | Jing Lv, Xiaodan Zhao, Yunhua Ye, Yawei Zhou, Huina Wang, Liyi Pan |
المصدر: | Chromatographia. 66:261-265 |
بيانات النشر: | Springer Science and Business Media LLC, 2007. |
سنة النشر: | 2007 |
مصطلحات موضوعية: | Hplc analysis, Chromatography, Chemistry, Organic Chemistry, Clinical Biochemistry, Central compartment, Biochemistry, High-performance liquid chromatography, Picroside I, Lower limit, Analytical Chemistry, Pharmacokinetics, Blood plasma, Quantitative analysis (chemistry) |
الوصف: | A sensitive and selective HPLC–UV method established for determination of picroside I in dog plasma has been used to study the pharmacokinetics of the drug after intravenous administration of three different doses. Sample pretreatment consists in deproteination by addition of acetonitrile; l-ascorbic acid was used to improve the stability of picroside I. The lower limit of quantification of picroside I was 0.05 μg mL−1. The recovery of the method was up to 90%. After intravenous administration to dogs picroside I was mainly distributed in the central compartment and was rapidly eliminated from the plasma; the mean elimination half-life was 30.54 ± 4.34, 30.20 ± 3.78, and 34.02 ± 1.88 min for doses of 2.5, 5, and 15 mg kg−1, respectively, and the respective values of AUC 0–∞ were 81.04 ± 19.95, 198.50 ± 27.77, and 586.44 ± 103.08 μg min mL−1. The different doses had no significant effect on the main pharmacokinetic data and the kinetics seemed to be linear in dosage range 2.5–15 mg kg−1. |
تدمد: | 1612-1112 0009-5893 |
URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::7f63fd955112a13c7fa0d3fad8106360 https://doi.org/10.1365/s10337-007-0288-5 |
رقم الأكسشن: | edsair.doi...........7f63fd955112a13c7fa0d3fad8106360 |
قاعدة البيانات: | OpenAIRE |
تدمد: | 16121112 00095893 |
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