Analysis of mtDNA mixtures from different fluids: An inter-laboratory study

التفاصيل البيبلوغرافية
العنوان: Analysis of mtDNA mixtures from different fluids: An inter-laboratory study
المؤلفون: S. Pagano, S. Sóñora, A. Di Lonardo, C. Cruz, M.C. Vide, M. López-Soto, A. Hernández, C.M. López-Cubría, Cristina Albarrán, Lourdes Prieto, R. Espinheira, Antonio Salas, M. Carbalho, Manuel Paredes, Martin R. Whittle, D.R. Sumita, M.J. Farfán, Vanesa Álvarez-Iglesias, J.A. Cano, J. Garcia-Hirchfeld, Daniel Corach, A. Alonso, M.F. Pinheiro, M. Crespillo, Gabriela Lima, A. Zurita, M. Montesino, S. Filippini, A. Sala
المصدر: International Congress Series. 1288:130-132
بيانات النشر: Elsevier BV, 2006.
سنة النشر: 2006
مصطلحات موضوعية: Genetics, Saliva, chemistry.chemical_compound, Mitochondrial DNA, chemistry, Haplotype, Semen, General Medicine, Inter-laboratory, Biology, Molecular biology, DNA, Hypervariable region
الوصف: The mitochondrial DNA (mtDNA) working group of the GEP-ISFG carried out an inter-laboratory exercise consisting of the study of mixture stains (saliva/semen and blood/semen) in order to investigate the behaviour of these common forensic samples when analysing their mtDNA using standard sequencing methodology. All labs extracted the DNA by preferential lysis and amplified and sequenced the first hypervariable region I (HVS-I). The results showed high consensus between labs for the first fraction of the lysis but not for the second one. We also observed differences between mixtures prepared from different donors and different body fluids. The present study has important consequences for the analysis and interpretation of mtDNA mixtures. © 2005 Elsevier B.V. All rights reserved.
تدمد: 0531-5131
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::7ff6176183b8846da1a302023608b83b
https://doi.org/10.1016/j.ics.2005.09.097
حقوق: CLOSED
رقم الأكسشن: edsair.doi...........7ff6176183b8846da1a302023608b83b
قاعدة البيانات: OpenAIRE