Reactive Oxygen Species and X-Ray Disrupted Spontaneous [Ca2+]iOscillation in Alveolar Macrophages
| العنوان: | Reactive Oxygen Species and X-Ray Disrupted Spontaneous [Ca2+]iOscillation in Alveolar Macrophages |
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| المؤلفون: | Zhi-Xiong Long, Feng Chen, Hong Ye, Qiong Zhou, Peng-Cheng Cai, Jian-Bao Xin, Hao Chen, Yang Jin, Wan-Li Ma, Da-Quan Meng, Xiao-Nan Tao, Li-Ping Zhu, Qiu Tang |
| المصدر: | Radiation Research. 179:485-492 |
| بيانات النشر: | Radiation Research Society, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | chemistry.chemical_classification, Reactive oxygen species, Radiation, Biophysics, Lung injury, Fluorescence, Molecular biology, Tetrandrine, Cytosol, chemistry.chemical_compound, chemistry, Biochemistry, In vivo, Radiology, Nuclear Medicine and imaging, Irradiation, Hydrogen peroxide |
| الوصف: | Radiation leads to a rapid burst of reactive oxygen species (ROS), which is considered to be one of the major causes of radiation-induced injury. ROS have previously been shown to induce changes in cytosolic Ca2+ ([Ca2+]i) including [Ca2+]i oscillation. However, the role of radiation in [Ca2+]i oscillation is poorly understood. The purpose of this study was to identify the effect of ROS and X ray on [Ca2+]i oscillation, as well as their role in radiation-induced lung injury. Alveolar macrophages were cultured in the absence and presence of different doses of hydrogen peroxide (H2O2) or exposed to X-ray irradiation with or without pretreatment of diphenyleneiodonium chloride (DPI, an inhibitor of NADPH oxidases) or tetrandrine (TET, a calcium entry blocker) and cytosolic Ca2+ concentration was detected by fluorescent Ca2+ indicator Fura-2. Rat radiation lung injury was induced in vivo by using 40 Gy X ray and DPI or TET was used to prevent radiation-induced lung injury. The results showed that there was sp... |
| تدمد: | 1938-5404 0033-7587 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::803d7c740ae843d9d00325528b3916a9 https://doi.org/10.1667/rr3006.1 |
| رقم الأكسشن: | edsair.doi...........803d7c740ae843d9d00325528b3916a9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19385404 00337587 |
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