A prognostic method for the litter size in Berkshire pigs based on DNA methylation of IGFBP4 gene
| العنوان: | A prognostic method for the litter size in Berkshire pigs based on DNA methylation of IGFBP4 gene |
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| المؤلفون: | Go Eun Yu, Tae Wan Kim, Jung Hye Hwang, Deok Gyeong Kang, Seulgi Kwon, Da Hye Park, Sang Mi An, Hwa Chun Park, Jeongim Ha, Chul Wook Kim |
| المصدر: | Canadian Journal of Animal Science. 98:845-851 |
| بيانات النشر: | Canadian Science Publishing, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Genetics, Litter (animal), 03 medical and health sciences, 030104 developmental biology, Food Animals, DNA methylation, Trait, Animal Science and Zoology, Epigenetics, Biology, Gene |
| الوصف: | Litter size is an important trait in the pig industry. Therefore, a lot of effort has been put into improving this trait. DNA methylation is an essential epigenetic modification present in unique DNA sequences. Alterations in methylation can affect transcription and phenotypic variation. The purpose of this study was to investigate the effect of DNA methylation on litter size. Methylation-specific restriction enzymes are simple and useful tools for detecting DNA methylation status. We used a pair of methylation-sensitive isoschizomers, which have the same recognition site, HpaII and MspI. Insulin-like growth factor binding protein 4 (IGFBP4) is a key regulator of ovarian follicular development and fetal growth in eutherian mammals. In this study, we discovered that IGFBP4 was hyper-methylated in the uterus tissue of a larger litter size group using bisulfite sequencing, and validated the positive relationship between the methylation status of IGFBP4 and the total number born of pigs using the porcine methylation-specific restriction enzyme polymerase chain reaction (PMP) assay. We suggest that the IGFPB4 gene can be used as a prognostic biomarker for hyperprolific sows and that the PMP assay is a useful tool for methylation status screening. |
| تدمد: | 1918-1825 0008-3984 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::841c2b3d8478a9c3584ff6adada8b6d2 https://doi.org/10.1139/cjas-2017-0160 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........841c2b3d8478a9c3584ff6adada8b6d2 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19181825 00083984 |
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