Strontium isotope analysis is a useful tool for tracing mobility and migration in past populations. For it to be employed, the 87Sr/86Sr values of the landscape must be well-understood. Bioavailable strontium is a combination of geological and atmospheric strontium available for use by plants and animals. In this study we begin mapping bioavailable strontium values around the Oldupai Gorge region so that this method may be utilized on archaeological hominins and animals in the future. We analyzed three plants from 33 localities across volcanic and metamorphic bedrock, including the regional drainage sump, Olbalbal. We found that bioavailable strontium in the region is homogeneous overall, with trends towards increasing values to the north and northeast and in Olbalbal. There was no difference between 87Sr/86Sr values of metamorphic and volcanic areas. Migrants from outside the study area with different isotopic values will be easily identifiable from the local residents.As a proof of concept, we analyzed 7 animal teeth (hippopotamus, crocodile, and equid) from Engaji Nanyori, a Bed III and IV site at Oldupai Gorge. We found that enamel and dentine which had been acetic acid treated to remove diagenetic strontium were significantly different from one another. All animals had higher 87Sr/86Sr values than the plant values, suggesting that modern and ancient bioavailable strontium values may have been different, likely due to environmental differences.