Novel vectors for retrotransposon-induced gene silencing in Magnaporthe oryzae
| العنوان: | Novel vectors for retrotransposon-induced gene silencing in Magnaporthe oryzae |
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| المؤلفون: | Masako Takino, Toshiki Murata, Hitoshi Nakayashiki, Ba Van Vu |
| المصدر: | Journal of General Plant Pathology. 77:147-151 |
| بيانات النشر: | Springer Science and Business Media LLC, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Genetics, Small interfering RNA, RNA silencing, RNA-induced transcriptional silencing, RNA-induced silencing complex, Trans-acting siRNA, Gene silencing, Plant Science, Argonaute, Biology, Agronomy and Crop Science, Gene |
| الوصف: | Here we report a novel system to induce RNA silencing in Magnaporthe oryzae using the retrotransposon MAGGY. The gene-silencing vector pSilent-MG carries a MAGGY variant with a unique cloning site in the 3′ UTR region and a geneticin-resistance cassette as a selection marker. A gene-silencing assay with a GFP gene revealed that pSilent-MG vectors induced gene silencing at a practical level. In the resulting GFP-silenced transformants, GFP siRNAs accumulated and DNA methylation increased in the GFP-expressing gene, implying that the vector could induce both transcriptional and post-transcriptional gene silencing. |
| تدمد: | 1610-739X 1345-2630 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::8bb53b307f470ed40b870eaa70fa39ca https://doi.org/10.1007/s10327-011-0302-5 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........8bb53b307f470ed40b870eaa70fa39ca |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 1610739X 13452630 |
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