Influence of environmental medium on membrane fatty acid composition of Reuber H35 hepatoma cells
| العنوان: | Influence of environmental medium on membrane fatty acid composition of Reuber H35 hepatoma cells |
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| المؤلفون: | Eduardo García-Peregrín, M. Carmen Garcia-Pelayo, Marina Martínez-Cayuela |
| المصدر: | Frontiers in Life Science. 7:123-131 |
| بيانات النشر: | Informa UK Limited, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | chemistry.chemical_classification, General Neuroscience, Fatty acid, Myristic acid, Lipid metabolism, Eicosapentaenoic acid, Lauric acid, General Biochemistry, Genetics and Molecular Biology, chemistry.chemical_compound, chemistry, Biochemistry, Membrane protein, Docosahexaenoic acid, lipids (amino acids, peptides, and proteins), Polyunsaturated fatty acid |
| الوصف: | Cell membrane proteins are sensitive to their fatty acid environment; however, the effects of those fatty acids are not completely understood. In this work, we have optimized a model for the study of membrane protein regulation, which allows the investigation of lipid metabolism in cultured tumor cells. The membrane fatty acid composition of Reuber H35 hepatoma cells was modified by enriching phospholipids in individual fatty acids of different length and degree of saturation. Lauric, myristic, eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids were supplemented to the culture medium of cells growing in presence or absence of lipoproteins. The four fatty acids were incorporated into membrane phospholipids in a dose-dependent manner. Lauric and myristic acids were actively metabolized, increasing percentages of longer and unsaturated chain fatty acids, but they did not modify the unsaturated index. As expected, EPA and DHA significantly decreased the unsaturated index. Incorporation of EPA and DHA was ... |
| تدمد: | 2155-3777 2155-3769 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::8d1993b50092054f0c9d03c2d02f75a8 https://doi.org/10.1080/21553769.2013.860406 |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........8d1993b50092054f0c9d03c2d02f75a8 |
| قاعدة البيانات: | OpenAIRE |
PDF full text from Publisher | تدمد: | 21553777 21553769 |
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