IL-17-induced Rgs16 expression is essential for follicular T helper cell localization in the germinal centers of autoimmune BXD2 mice (123.44)
| العنوان: | IL-17-induced Rgs16 expression is essential for follicular T helper cell localization in the germinal centers of autoimmune BXD2 mice (123.44) |
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| المؤلفون: | Yanna Ding, Hui-Chen Hsu, Kirk Druey, Qi Wu, Jun Li, PingAr Yang, Allan Zajac, John Mountz |
| المصدر: | The Journal of Immunology. 188:123.44-123.44 |
| بيانات النشر: | The American Association of Immunologists, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Immunology, Immunology and Allergy |
| الوصف: | Autoimmune BXD2 mice developed spontaneous germinal centers (GCs) and displayed increased number of CXCR5+ICOS+follicular T helper (Tfh) cells in the spleen. Although IL-21 but not IL-17 has been reported to be essential for the development of Tfh, there were significantly reduced PNA+Fas+GC B cells in the spleen of BXD2-Il21-/- and BXD2-Il17ra-/- mice. Interestingly, the frequency of Tfhs was 2.5-fold lower in BXD2-Il21-/- but 1.5-fold higher in BXD2-Il17ra-/- mice compared to wild type. Although increased, CXCR5+CD4 T cells were not specifically localized to the GCs in BXD2-Il17ra-/- spleens. Administration of AdIL-21 increased IL-17 levels in Tfhs and serum but had minimal effect on GC B cell frequencies in BXD2-Il17ra-/- spleens. In contrast, administration of AdIL-17 to BXD2-Il21-/- mice partially expanded GC B cells, suggesting that IL-17 acts downstream of IL-21 to facilitate the GC response. IL-21 stimulation of spleen CD4 T cells in vitro increased the frequency of Tfhs and upregulated expression of IL17R on Tfhs. IL-17 stimulated expression of Rgs16 and diminished CXCL13-mediated chemotaxis of CD4 T cells in vitro. Consistent with this, there were fewer GC CD4 T cells in BXD2-Rgs16-/- spleens in vivo. Our study suggests that IL-21 and IL-17 are required to promote the maximal autoreactive GC responses. IL-21 is important for Tfh development whereas IL-17 induced Rgs16 expression is required for Tfh localization in GCs to promote GC B-cell development. |
| تدمد: | 1550-6606 0022-1767 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::8d3d842a61825a77ab18fe63f14cc03b https://doi.org/10.4049/jimmunol.188.supp.123.44 |
| رقم الأكسشن: | edsair.doi...........8d3d842a61825a77ab18fe63f14cc03b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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