Chemical modification of E. coli L ‐asparaginase with polyethylene glycol grafted vinylpyrrolidone–maleic anhydride copolymer
| العنوان: | Chemical modification of E. coli |
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| المؤلفون: | Jianbiao Ma, Guoqiang Qian, Daobin Wang, Juyan Zhou, Binglin He |
| المصدر: | Polymers for Advanced Technologies. 8:581-586 |
| بيانات النشر: | Wiley, 1997. |
| سنة النشر: | 1997 |
| مصطلحات موضوعية: | Antigenicity, Materials science, Polymers and Plastics, Maleic acid, biology, Chemical modification, Maleic anhydride, Polyethylene glycol, Trypsin, Enzyme assay, chemistry.chemical_compound, chemistry, Polymer chemistry, medicine, Copolymer, biology.protein, medicine.drug |
| الوصف: | A series of polyethylene glycol grafted vinylpyrrolidone–maleic anhydride copolymers [P(VMP)] was synthesized by radical copolymerization of vinylpyrrolidone, maleic anhydride and polyethylene glycol maleic acid monoester. Escherichia coli l-asparaginase was chemically modified with these copolymers. The modified l-asparaginase exhibited the complete loss of antigenicity towards anti-asparaginase serum from rabbit. The highest enzyme activity of the modified l-asparaginase without antigenicity was retained by 59% of the nonmodified one. The modified enzyme was also more resistant to trypsin in vitro. When tested in vivo, the native l-asparaginase was quickly cleared from the plasma of rabbits (half-life time: t1/2=1.2 hr), whereas the modified enzyme showed prolonged clearance from plasma (t1/2=53 hr). © 1997 John Wiley & Sons, Ltd. |
| تدمد: | 1099-1581 1042-7147 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::8fbe285efa03e28514f37dd0c6f180cd https://doi.org/10.1002/(sici)1099-1581(199710)8:10<581::aid-pat689>3.0.co;2-# |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........8fbe285efa03e28514f37dd0c6f180cd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10991581 10427147 |
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