Investigation of extracellular matrix genes associated with Alzheimer’s disease in the hippocampus of experimental diabetic model rats
| العنوان: | Investigation of extracellular matrix genes associated with Alzheimer’s disease in the hippocampus of experimental diabetic model rats |
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| المؤلفون: | Isil Ocal, M. Bertan Yılmaz, Hülya Binokay, Lütfiye Özpak, Ayfer Pazarbaşı |
| المصدر: | International Journal of Diabetes in Developing Countries. 42:82-90 |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | medicine.medical_specialty, endocrine system diseases, business.industry, Endocrinology, Diabetes and Metabolism, Insulin tolerance test, nutritional and metabolic diseases, 030209 endocrinology & metabolism, Type 2 diabetes, Streptozotocin, medicine.disease, Pathophysiology, Extracellular matrix, 03 medical and health sciences, 0302 clinical medicine, Endocrinology, Internal medicine, Diabetes mellitus, Gene expression, Internal Medicine, medicine, Hippocampus (mythology), 030212 general & internal medicine, business, medicine.drug |
| الوصف: | Both type 2 diabetes (T2D) and Alzheimer’s disease affect large number of people all over the world, especially in developed countries, and involve common molecular mechanisms in degenerative processes. Our study is to investigate the expression levels of the ADAMTS4, TIMP3, RELN, and BCAN genes which encode extracellular matrix molecules and are thought to be related to the pathophysiology of Alzheimer’s disease in rats that we used to develop a T2D model by injection of a streptozotocin (STZ) and feeding with high-fat diet (HFD). In total, 40 rats were divided into four groups: HFD + STZ (10), HFD (10), STZ (10), and the control (10). The weight and blood glucose levels of all rats were recorded, and the insulin tolerance test was performed. At the end of the experimental period, the hippocampus of the rats was isolated and a part of this was used for gene expression analysis through real-time PCR, whereas other parts were used for histological analyses. Our results show that plaque-like structures were found in the histological examination of the experimental T2D model. In molecular studies, the expression levels of the ADAMTS4, TIMP3, RELN, and BCAN genes were decreased in the HFD +STZ and STZ groups compared with the control, whereas the same expression levels, except that of inhibitor TIMP3, were found to increase in the HFD group. These genes encoding proteases that regulate neuronal activity have decreased levels in the T2D model. There may be potential in the adoption of new treatment approaches for Alzheimer’s and T2D. |
| تدمد: | 1998-3832 0973-3930 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::92993bbe74121c55009edf16047907e3 https://doi.org/10.1007/s13410-021-00951-7 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........92993bbe74121c55009edf16047907e3 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 19983832 09733930 |
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