Combination of let-7d-5p, miR-26a-5p, and miR-15a-5p is suitable normalizer for studying microRNA expression in skin tissue of Liaoning cashmere goat during hair follicle cycle
| العنوان: | Combination of let-7d-5p, miR-26a-5p, and miR-15a-5p is suitable normalizer for studying microRNA expression in skin tissue of Liaoning cashmere goat during hair follicle cycle |
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| المؤلفون: | R. L. Yin, J. J. Wang, R. H. Yin, Y. B. Zhu, W. L. Bai, W. Q. Jiang, Y. L. Dang, G. B. Luo, Z. Y. Wang, Z. H. Zhao |
| المصدر: | Czech Journal of Animal Science. 61:99-107 |
| بيانات النشر: | Czech Academy of Agricultural Sciences, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, RNA, Ribosomal RNA, Biology, Hair follicle, Cell biology, 03 medical and health sciences, 030104 developmental biology, medicine.anatomical_structure, Botany, microRNA, medicine, Cashmere goat, Animal Science and Zoology, Small nucleolar RNA, Gene, Small nuclear RNA |
| الوصف: | The microRNAs are non-coding RNA molecules of approximately 20–22 nucleotides that are found to be implicated in a wide range of physiological processes. In this study, the suitability of 10 candidate reference RNAs was evaluated for microRNA expression data in the skin tissue of Liaoning cashmere goat including 1 small nuclear RNA (snRNA; RNU6B), 1 small nucleolar RNA (snoRNA; Z30), 1 rRNA (5S), 1 transfer RNA (tRNA; MettRNA), and 6 microRNAs (miR; let-7d-5p, miR-15a-5p, miR-26a-5p, miR-125a-5p, miR-214-3p, and miR-221-3p). Based on geNorm and NormFinder algorithms, we identified let-7d-5p, miR-26a-5p, and miR-15a-5p as the most stable reference RNAs. Also, three reference RNAs (let-7d-5p, miR-26a-5p, and miR-15a-5p) were sufficient for the normalization of microRNA expression data in the skin of this breed. We further assessed the suitability of let-7d-5p, miR-26a-5p, and miR-15a-5p in a combination as reference RNAs through detecting the relative expression of miR-24-3p, miR-29a-3p, miR-145a-5p, and miR-205-5p as putative genes of interest. Significant differences were revealed in the relative expression of miR-24-3p, miR-29a-3p, miR-145a-5p, and miR-205-5p at telogen stage of hair follicle cycle when a combination of let-7d-5p, miR-26a-5p, and miR-15a-5p vs a single let-7d-5p were used as reference RNA. Based on the results from this study, we suggested that the combination of let-7d-5p, miR-26a-5p, and miR-15a-5p as normalizers for microRNA expression data would be more reliable than that of single let-7d-5p, and the geometric mean of these three microRNAs (let-7d-5p, miR-26a-5p, and miR15a-5p) can be used for the normalization of microRNAs expression data in the skin of Liaoning cashmere goat. |
| تدمد: | 1805-9309 1212-1819 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::956ad7c155af108546d54cad66ae6ad1 https://doi.org/10.17221/8782-cjas |
| حقوق: | OPEN |
| رقم الأكسشن: | edsair.doi...........956ad7c155af108546d54cad66ae6ad1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18059309 12121819 |
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