Two small linear plasmids of Streptomyces jumonjinensis
| العنوان: | Two small linear plasmids of Streptomyces jumonjinensis |
|---|---|
| المؤلفون: | Kenneth L. Roy, Donald J. Netolitzky, Susan E. Jensen |
| المصدر: | Canadian Journal of Microbiology. 43:633-638 |
| بيانات النشر: | Canadian Science Publishing, 1997. |
| سنة النشر: | 1997 |
| مصطلحات موضوعية: | Genetics, biology, Streptomycetaceae, Immunology, General Medicine, biology.organism_classification, Applied Microbiology and Biotechnology, Microbiology, Streptomyces, Homology (biology), Streptomyces jumonjinensis, Plasmid, Restriction map, Actinomycetales, Molecular Biology, Bacteria |
| الوصف: | In a survey of plasmids in a variety of β-lactam antibiotic producing Streptomyces spp., two small linear plasmids (pSJL1 and pSJL2) of approximately 12 and 17.5 kb were detected within Streptomyces jumonjinensis NRRL 5741, in addition to the previously reported giant linear plasmids pSJL3 and pSJL4. Characterization of these plasmids by Southern hybridization indicated that no significant homology exists between the S. jumonjinensis plasmids and plasmids detected in other β-lactam antibiotic producing Streptomyces spp. Single and double restriction endonuclease digestions were performed to generate maps of the two plasmids. The plasmids pSJL1 and pSJL2 have copy numbers of 21–27 and 15–20, respectively.Key words: Streptomyces, linear plasmid, DNA hybridization, DNA homology. |
| تدمد: | 1480-3275 0008-4166 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::980f0f5386c443a3cff40b711d004f9e https://doi.org/10.1139/m97-090 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........980f0f5386c443a3cff40b711d004f9e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14803275 00084166 |
|---|