Super-Resolving the Distance-Dependent Plasmon-Enhanced Fluorescence of Single Dye and Fluorescent Protein Molecules
| العنوان: | Super-Resolving the Distance-Dependent Plasmon-Enhanced Fluorescence of Single Dye and Fluorescent Protein Molecules |
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| المؤلفون: | Bing Fu, Benjamin P. Isaacoff, David J. Rowland, Jessica D. Flynn, Julie S. Biteen |
| المصدر: | The Journal of Physical Chemistry C. 119:19350-19358 |
| بيانات النشر: | American Chemical Society (ACS), 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Quenching (fluorescence), Chemistry, Fluorescence in the life sciences, Photochemistry, Fluorescence, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, General Energy, Quantum efficiency, Physical and Theoretical Chemistry, mCherry, Biological imaging, Plasmon, Fluorescence loss in photobleaching |
| الوصف: | Coupling to metal nanoparticles can increase the fluorescence intensity and photostability of fluorescent probes, and this plasmon-enhanced fluorescence is particularly promising for the dimmer fluorescent proteins common in biological imaging. Here, we measure the intensity distribution of single Cy3.5 dye molecules and mCherry fluorescent proteins one at a time as they adsorb on a conformal surface 4.8–61.0 nm thick over a gold nanorod (NR). The emission intensities for both types of fluorophores depend nonmonotonically on the spacer thickness, and an optimal spacer thickness of ∼10 nm is observed for both fluorophores using two different spacer layer materials. Emission from fluorophores coupled to metal nanoparticles is affected by two competing processes: an enhanced spontaneous decay rate and quenching via nonradiative antenna modes. After averaging over a conformal surface, the product of the simulated enhanced local electric field intensity and the quantum efficiency modification reproduces the ex... |
| تدمد: | 1932-7455 1932-7447 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::9cf2a50e6f9ab8008420b211efc9dc58 https://doi.org/10.1021/acs.jpcc.5b05154 |
| رقم الأكسشن: | edsair.doi...........9cf2a50e6f9ab8008420b211efc9dc58 |
| قاعدة البيانات: | OpenAIRE |
Find this issue from the American Chemical Society | تدمد: | 19327455 19327447 |
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