Turnera subulata plant samples were exhaustively extracted through continuous hot percolation method by selective sequential soxhlet extraction using solvents of increasing polarity. All the extracts were subjected to qualitative analysis to identify, analyse and characterize the bioactive phytocompounds present in each extract, about which much information are unavailable. The qualitative preliminary phytochemical, FT-IR (Fourier-transform infrared spectroscopy) and GC–MS (Gas chromatography–mass spectrometry) analyses showed the presence of numerous novel pharmacologically important phytocompounds. Based on qualitative studies, chloroform, ethyl acetate and ethanol extracts of T. subulata have chosen for LC–MS/MS analysis to identify polyphenols; the total 21 compounds included 6 flavonols, 1 dihydroflavonols, 4 flavonones, 2 flavones, 3 anthocyanins, 2 isoflavonoids, 2 phenolic acids, and 1 hydroxybenzaldehyde. The above mentioned extracts showed good antibacterial and antifungal effects against the pathogenic bacteria (Escherichia coli and Staphylococcus aureus) and fungi (Aspergillus niger and Candida albicans). In addition, the crude plant extracts showed significant physicochemical properties, which might be attributed to the significant quantity of compounds present in T. subulata. Current study revealed that T. subulata plant extracts possessed novel bioactive phytocompounds and thus, offered a varied platform for further use of the plant as herbal alternative for various ailments.
