PP2C.D phosphatase SAL1 positively regulates aluminum resistance via restriction of aluminum uptake in rice
| العنوان: | PP2C.D phosphatase SAL1 positively regulates aluminum resistance via restriction of aluminum uptake in rice |
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| المؤلفون: | Wenxiang Xie, Shuo Liu, Huiling Gao, Jun Wu, Dilin Liu, Toshinori Kinoshita, Chao-Feng Huang |
| المصدر: | Plant Physiology. 192:1498-1516 |
| بيانات النشر: | Oxford University Press (OUP), 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Physiology, Genetics, Plant Science |
| الوصف: | Aluminum (Al) toxicity represents a primary constraint for crop production in acidic soils. Rice (Oryza sativa) is a highly Al-resistant species; however, the molecular mechanisms underlying its high Al resistance are still not fully understood. Here, we identified SAL1 (SENSITIVE TO ALUMINUM 1), which encodes a plasma membrane (PM)-localized PP2C.D phosphatase, as a crucial regulator of Al resistance using a forward genetic screen. SAL1 was found to interact with and inhibit the activity of PM H+-ATPases, and mutation of SAL1 increased PM H+-ATPase activity and Al uptake, causing hypersensitivity to internal Al toxicity. Furthermore, knockout of NRAT1 (NRAMP ALUMINUM TRANSPORTER 1) encoding an Al uptake transporter in a sal1 background rescued the Al-sensitive phenotype of sal1, revealing that coordination of Al accumulation in the cell, wall and symplasm is critical for Al resistance in rice. By contrast, we found that mutations of PP2C.D phosphatase-encoding genes in Arabidopsis (Arabidopsis thaliana) enhanced Al resistance, which was attributed to increased malate secretion. Our results reveal the importance of PP2C.D phosphatases in Al resistance and the different strategies used by rice and Arabidopsis to defend against Al toxicity. |
| تدمد: | 1532-2548 0032-0889 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::9ec0417ad205252928771148b9e8ca0a https://doi.org/10.1093/plphys/kiad122 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........9ec0417ad205252928771148b9e8ca0a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15322548 00320889 |
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