Biologically synthesized silver nanoparticles, mediated by Bothriochloa laguroides , inhibit biofilm formation and eradicate mature biofilm of Yersinia enterocolitica and Staphylococcus aureus
| العنوان: | Biologically synthesized silver nanoparticles, mediated by Bothriochloa laguroides , inhibit biofilm formation and eradicate mature biofilm of Yersinia enterocolitica and Staphylococcus aureus |
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| المؤلفون: | Cecilia Lucero-Estrada, María Gabriela Ortega, Paulina Laura Páez, Pamela Soledad Bustos, Araceli Toranzo |
| المصدر: | Journal of Applied Microbiology. 132:209-220 |
| بيانات النشر: | Oxford University Press (OUP), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | biology, Chemistry, Biofilm, General Medicine, medicine.disease_cause, biology.organism_classification, Applied Microbiology and Biotechnology, Silver nanoparticle, Microbiology, Bothriochloa laguroides, Proanthocyanidin, Phytochemical, Staphylococcus aureus, medicine, Antibacterial activity, Yersinia enterocolitica, Biotechnology |
| الوصف: | AIMS To phytosynthesize silver nanoparticles (AgNPs) and determine their antibacterial and antibiofilm capacity against gram-positive and gram-negative bacterial strains. METHODS AND RESULTS AgNPs were synthesized using Bothriochloa laguroides aqueous extract as reducing and stabilizing agent. After characterization, a phytochemical screening to the extract and the AgNPs was performed. Antibacterial activity, inhibition and eradication of biofilms against Staphylococcus aureus and Yersinia enterocolitica strains were tested. Spherical AgNPs with an average size of 8 nm were obtained. Tannins, flavonoids, carbohydrates, proanthocyanidins, anthocyanins and saponins were identified in aqueous extract; meanwhile, only carbohydrates were identified in AgNPs. The MIC and MBC were determined at pmol L-1 levels for all tested strains. Furthermore, AgNPs inhibited more than 90% of biofilms formation and eradicated more than 80% of mature biofilms at concentrations higher than MIC. CONCLUSIONS The AgNPs obtained in this study inhibited planktonic and sessile growth, and eradicated mature biofilms of pathogenic bacterial strains at very low concentrations. SIGNIFICANCE AND IMPACT OF STUDY The current study showed the promising potential of AgNPs as antibiofilm agents opening the way for the future development of a new class of antibacterial products. |
| تدمد: | 1365-2672 1364-5072 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::9f2d32c29fa3c2ce7a301fad815ef08a https://doi.org/10.1111/jam.15195 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........9f2d32c29fa3c2ce7a301fad815ef08a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13652672 13645072 |
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