Optimal Fermentation of Saccharomyces cerevisiae Expressing a Dextranase from Chaetomium gracile
| العنوان: | Optimal Fermentation of Saccharomyces cerevisiae Expressing a Dextranase from Chaetomium gracile |
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| المؤلفون: | Hongbei Wei, Jidong Liu, Fangxue Hang, Hang Yin, Kai Li, Qinju Sun, Leyi Wang |
| المصدر: | Sugar Tech. 22:171-178 |
| بيانات النشر: | Springer Science and Business Media LLC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | 0106 biological sciences, Shake flask, Dextranase, biology, Chemistry, Sugar industry, Saccharomyces cerevisiae, 04 agricultural and veterinary sciences, Chaetomium gracile, biology.organism_classification, 01 natural sciences, Dextranase activity, law.invention, law, 040103 agronomy & agriculture, Recombinant DNA, 0401 agriculture, forestry, and fisheries, Fermentation, Food science, Agronomy and Crop Science, 010606 plant biology & botany |
| الوصف: | Dextranase from Chaetomium gracile is generally considered safe for use in the sugarcane industry. Herein, a truncated and codon-optimised α-dextranase gene from C. gracile was successfully cloned and expressed in Saccharomyces cerevisiae for the first time. The optimum conditions of fermentation was achieved when the maximum dextranase activity reached to 58.45 U/mL after 48 h in shake flasks. The optimal pH and temperature were 5.5 and 60 °C, respectively. The recombinant dextranase remained stable between pH 4 and 6 and temperature between 55 and 60 °C. The findings in the present study could facilitate large-scale production of food-grade recombinant dextranase for use in the sugar industry. |
| تدمد: | 0974-0740 0972-1525 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::a0b95b51a494bb6a8515392248d81762 https://doi.org/10.1007/s12355-019-00746-5 |
| حقوق: | CLOSED |
| رقم الأكسشن: | edsair.doi...........a0b95b51a494bb6a8515392248d81762 |
| قاعدة البيانات: | OpenAIRE |
Find this article in full text from Springer Verlag. | تدمد: | 09740740 09721525 |
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