A Novel Suspended Hydrogel Membrane Platform for Cell Culture
| العنوان: | A Novel Suspended Hydrogel Membrane Platform for Cell Culture |
|---|---|
| المؤلفون: | Shihao Yang, Pranav Soman, Jessica L. Ouderkirk, Yong X. Chen, Jiahan Yan, Lingyan Weng, Mira Krendel, Ming Han Hsieh |
| المصدر: | Journal of Nanotechnology in Engineering and Medicine. 6 |
| بيانات النشر: | ASME International, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Materials science, food.ingredient, Petri dish, Nanotechnology, General Medicine, Matrix (biology), Gelatin, law.invention, Membrane, food, In vivo, Cell culture, law, Cancer cell, Self-healing hydrogels, General Materials Science, Electrical and Electronic Engineering, Biomedical engineering |
| الوصف: | Current cell-culture is largely performed on synthetic two-dimensional (2D) petri dishes or permeable supports such as Boyden chambers, mostly because of their ease of use and established protocols. It is generally accepted that modern cell biology research requires new physiologically relevant three-dimensional (3D) cell culture platform to mimic in vivo cell responses. To that end, we report the design and development of a suspended hydrogel membrane (ShyM) platform using gelatin methacrylate (GelMA) hydrogel. ShyM thickness (0.25–1 mm) and mechanical properties (10–70 kPa) can be varied by controlling the size of the supporting grid and concentration of GelMA prepolymer, respectively. GelMA ShyMs, with dual media exposure, were found to be compatible with both the cell-seeding and the cell-encapsulation approach as tested using murine 10T1/2 cells and demonstrated higher cellular spreading and proliferation as compared to flat GelMA unsuspended control. The utility of ShyM was also demonstrated using a case-study of invasion of cancer cells. ShyMs, similar to Boyden chambers, are compatible with standard well-plates designs and can be printed using commonly available 3D printers. In the future, ShyM can be potentially extended to variety of photosensitive hydrogels and cell types, to develop new in vitro assays to investigate complex cell–cell and cell–extracellular matrix (ECM) interactions. |
| تدمد: | 1949-2952 1949-2944 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::a0c6c9b6e9b608121a29c148f4d8d320 https://doi.org/10.1115/1.4031467 |
| رقم الأكسشن: | edsair.doi...........a0c6c9b6e9b608121a29c148f4d8d320 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19492952 19492944 |
|---|